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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Intra‐articular Injection of platelet‐rich fibrin releasates in combination with bone marrow‐derived mesenchymal stem cells in the treatment of articular cartilage defects: An in vivo in vivo study in rabbits
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Intra‐articular Injection of platelet‐rich fibrin releasates in combination with bone marrow‐derived mesenchymal stem cells in the treatment of articular cartilage defects: An in vivo in vivo study in rabbits

机译：关节内注射富含血小板的纤维蛋白与骨髓衍生的间充质干细胞组合释放在治疗关节软骨缺陷中：在兔体内体内体内研究

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Abstract The use of mesenchymal stem cells (MSCs), which can be differentiated into chondrocytes under specific conditions, has been proposed for the treatment of cartilage defects. Blood‐derived platelet‐rich fibrin releasate (PRFr), which is rich in growth factors and cytokines, may improve cartilage regeneration. In this study, the therapeutic effects of PRFr in combination with bone marrow‐derived MSCs for articular cartilage regeneration were evaluated in a rabbit model. Critical osteochondral defects were surgically created in the femoral condyle of the rabbits, and 3 × 10 6 of MSCs, 0.8 mL of PRFr, or a combination of MSCs and PRFr were injected intra‐articularly and one week after first administration. The animals were sacrificed 12 weeks postoperatively, and the regenerated cartilages were assessed by gross inspection and histological examination. No treatment‐related adverse events were noted in any of the rabbits. The size of the defect decreased and the volume of regenerated cartilage increased in the medial femoral condyles of the MSCs?+?PRFr group. Relative to the MSCs or PRFr group, histological examination demonstrated that the MSCs?+?PRFr group had thicker hyaline‐like cartilaginous tissue with normal glycosaminoglycan production. Grading scores revealed that MSCs?+?PRFr injection had better matrix, cell distribution, and surface indices than other groups. The results showed that intra‐articular injections of MSCs?+?PRFr into the knee can reduce cartilage defects by regenerating hyaline‐like cartilage without adverse events. This approach may provide an alternative method of autologous chondrocyte implantation to repair cartilage defects with an unlimited source of cells and releasate. ? 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1536–1543, 2017.
机译：摘要已经提出了在特定条件下，可以在特定条件下分化成软骨细胞的间充质干细胞（MSCs）用于治疗软骨缺陷。富含血小板的富含血小板纤维蛋白（PRFR），其富含生长因子和细胞因子，可改善软骨再生。在该研究中，在兔模型中评估PRFR与骨髓衍生的MSCs组合的治疗效果。在兔子的股骨髁上外科造成临界骨质缺损，并且在第一施用后，3×10 6的MSC，0.8mL的PRFR或MSC和PRFR的组合注射，并且在第一施用后一周内注射。术后12周被处死动物，通过总检测和组织学检查评估再生软骨。任何兔子都没有注意到治疗相关的不良事件。缺陷的大小降低，再生软骨的体积在MSC的内侧股骨髁上增加了α+αα·普遍组。相对于MSCs或PRFR组，组织学检查表明MSCS？+α+α+α+β+α+β+α+β般的透明状软骨组织含有正常的糖胺聚糖生产。评分分数显示MSCS？+？PRFR注射具有比其他组的更好的矩阵，细胞分布和表面指数。结果表明，关节内注射MSC？+α+膝关节可以通过再生透明的软骨，而没有不良事件，可以减少软骨缺陷。该方法可以提供自体软骨细胞植入的替代方法，以修复用无限制的细胞来源和释放的软骨缺陷。还2016 Wiley期刊，Inc.J生物保解员B部分B：Appl Biomater，105B：1536-1543,2017。

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