Lipase production by solid‐state fermentation of olive pomace in tray‐type and pressurized bioreactors

Oliveira Felisbela; Salgado José Manuel; Pérez‐Rodríguez Noelia; Domínguez José Manuel; Venancio Armando; Belo Isabel

摘要

Abstract <section xml:id="jctb5492-sec-0001" numbered="no"> <title type="main">BACKGROUND >Bioreactor type, sterilization and specific operational conditions are key factors for the scale‐up of solid‐state fermentation (SSF). This work deals with the lipase production by SSF of olive pomace (OP) in traditional tray‐type and pressurized bioreactors. Important aspects of SSF in bioreactors were studied, such as the need for sterilization and moisture content (MC) control. </section> <section xml:id="jctb5492-sec-0002" numbered="no"> <title type="main">RESULTS > At larger scale, there was no significant difference in lipase production between sterilized and unsterilized substrates, but MC control had significant impact. The production of lipase in a pressurized bioreactor, under air absolute pressure of 200 kPa and 400 kPa, was 2‐fold higher than in a tray‐type bioreactor using the same amount of substrate (500 g) and the same bed height. The protein content of substrate increased from 10 to 18% (w/w) after SSF and the fermented solid presented an antioxidant activity of 10 mmol Trolox kg ‐1 . </section> <section xml:id="jctb5492-sec-0003" numbered="no"> <title type="main">CONCLUSIONS >SSF in a pressurized bioreactor enabled efficient production of lipase with higher substrate bed height in contrast to that in a tray‐type bioreactor. The improvement in nutritional value of the substrate by SSF indicates its potential applicability in animal feed. ? 2017 Society of Chemical Industry </section> </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“JCTB5492-SEC-0001”编号=“否”> <标题类型=“main”>背景</ title> >生物反应器类型，灭菌和特定操作条件是固态扩展的关键因素发酵（SSF）。这项工作涉及通过传统托盘型和加压生物反应器的橄榄渣（OP）SSF的脂肪酶生产。研究了生物反应器中SSF的重要方面，例如对灭菌和水分含量（MC）控制的需求。</ p> </段> <章节XML：ID =“JCTB5492-SEC-0002”编号=“否”> <标题类型=“main”>结果</标题> >在较大刻度下，灭菌和烯化基材之间的脂肪酶生产没有显着差异，但MC控制产生了显着的影响。在200kPa和400kPa的空气绝对压力下，在加压生物反应器中的脂肪酶的产生比使用相同量的基材（500g）和相同的床高度在托盘型生物反应器中高2倍。 SSF后，基材的蛋白质含量增加到10至18％（w / w），并且发酵固体呈现10mmol roloLox kg -1 / sup>的抗氧化活性。 </ p> </ section> <section XML：ID =“JCTB5492-SEC-0003”编号=“否”> <标题类型=“main”>结论</ title> > SSF在加压生物反应器中启用了高效与托盘型生物反应器相比，脂肪酶高度的脂肪酶的生产。 SSF底物营养价值的改善表明其在动物饲料中的潜在适用性。还2017年化学工业协会</ p> </段> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第5期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Oliveira Felisbela&option=202" target="_blank" rel="nofollow">Oliveira Felisbela;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Salgado José Manuel&option=202" target="_blank" rel="nofollow">Salgado José Manuel;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pérez‐Rodríguez Noelia&option=202" target="_blank" rel="nofollow">Pérez‐Rodríguez Noelia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Domínguez José Manuel&option=202" target="_blank" rel="nofollow">Domínguez José Manuel;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Venancio Armando&option=202" target="_blank" rel="nofollow">Venancio Armando;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Belo Isabel&option=202" target="_blank" rel="nofollow">Belo Isabel;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>CEB – Centre of Biological EngineeringUniversity of MinhoBraga Portugal;</p> <p>CEB – Centre of Biological EngineeringUniversity of MinhoBraga Portugal;</p> <p>Department of Chemical EngineeringUniversity of VigoOurense Spain;</p> <p>Department of Chemical EngineeringUniversity of VigoOurense Spain;</p> <p>CEB – Centre of Biological EngineeringUniversity of MinhoBraga Portugal;</p> <p>CEB – Centre of Biological EngineeringUniversity of MinhoBraga Portugal;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=solid‐state fermentation&option=203" rel="nofollow">solid‐state fermentation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=olive pomace&option=203" rel="nofollow">olive pomace;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lipase production&option=203" rel="nofollow">lipase production;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=tray‐type bioreactor&option=203" rel="nofollow">tray‐type bioreactor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=pressurized bioreactor&option=203" rel="nofollow">pressurized bioreactor;</a> </p> <div class="translation"> 机译：固态发酵;橄榄渣;脂肪酶生产;托盘型生物反应器;加压生物反应器; 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