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首页> 外文期刊>Journal of genetics >Sequence diversity of MHC class-II DRB gene in gazelles (Gazella subgutturosa) raised in Sanliurfa of Turkey
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Sequence diversity of MHC class-II DRB gene in gazelles (Gazella subgutturosa) raised in Sanliurfa of Turkey

机译：在土耳其的Sanliurfa中升起的瞪羚（Gazella subgutturosa）中MHC级DRB基因的序列多样性

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摘要

In this study, we aimed to assess the sequence diversity of major histocompatibility complex (MHC) class-II DRB gene at exon 2 in gazelles raised in Sanliurfa Province of Turkey. Twenty DNA samples isolated from gazelles (Gazella subgutturosa) were used for sequencing exon 2 of MHC class-II DRB gene. Target region was amplified by polymerase chain reaction (PCR) and their products were directly sequenced. Nine of these 20 samples yielded unambiguously readable sequences. Three of the nine samples were homozygotes and each showed different sequences. A 262-bp sequence obtained from the three homozygote samples were submitted to GenBank (accession numbers: KC309405, KC309406 and KC309407). Using an allele specific PCR, we detected 10 additional haplotypes. Among 13 haplotypes, 45 nucleotide positions were polymorphic and most of the polymorphic nucleotide positions localized at peptide-binding region (PBR). Rates of nonsynonymous substitutions were significantly higher than synonymous substitutions at PBR. Phylogenetic analysis of the haplotypes showed that 10 haplotypes of the gazelles were clustered together while three were clustered with ovine and bovine haplotypes. The results indicated that at least 13 haplotypes at exon 2 of MHC class-II DRB gene were showing high degree of nucleotide and amino acid diversity, and certain haplotypes of G. subgutturosa were more similar to haplotypes from sheep or cattle than to each other. Rates of synonymous and nonsynonymous substitutions suggested that positive selection was a driving force for diversity at this locus in G. subgutturosa.
机译：在这项研究中，我们旨在评估在土耳其三尔法省省瞪羚的外显子2的主要组织相容性综合体（MHC）II类DRB基因的序列多样性。从瞪羚（Gazella subgutturosa）中分离的二十个DNA样本用于MHC-II DRB基因的测序2。通过聚合酶链反应（PCR）扩增靶区域，并直接测序它们的产物。这20个样品中的九个产生明确可读的序列。九个样品中的三个是纯合子，每个序列都显示出不同的序列。将三种纯合子样品获得的262bp序列提交至Genbank（登录号：KC309405，KC309406和KC309407）。使用等位基因特异性PCR，我们检测到10个额外的单倍型。在13个单倍型中，45个核苷酸位置是多态性的，并且大多数在肽结合区域（PBR）定位的多态性核苷酸位置。非纯代级取代的速率明显高于PBR的同义取代。单倍型的系统发育分析表明，凝胶凝胶的10个单倍型聚集在一起，而三个与绵羊和牛单倍型聚集。结果表明，MHC II类DRB基因的外显子2的至少13个单倍型显示出高度的核苷酸和氨基酸多样性，并且G.Suputturosa的某些单倍型更类似于来自绵羊或牛的单倍型而不是彼此的单倍型。同义词和非唯一替换的速率表明，正面选择是G. SubGutturosa中该基因座的多样性的驱动力。

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