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首页> 外文期刊>Journal of mass spectrometry: JMS >Liquid microjunction surface sampling probe electrospray mass spectrometry for detection of drugs and metabolites in thin tissue sections
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Liquid microjunction surface sampling probe electrospray mass spectrometry for detection of drugs and metabolites in thin tissue sections

机译：液体微型表面采样探针电喷雾质谱法检测薄组织切片中的药物和代谢物

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摘要

A self-aspirating, liquid microjunction surface sampling probe/electrospray emitter mass spectrometry. system was demonstrated for use in the direct analysis of spotted and dosed drugs and their metabolites in thin tissue sections. Proof-of-principle sampling and analysis directly from tissue without the need for sample preparation was demonstrated first by raster scanning a region on a section of rat liver onto which reserpine was spotted. The mass spectral signal from selected reaction monitoring was used to develop a chemical image of the spotted drug on the tissue. The probe was also used to selectively spot sample areas of sagittal whole-body tissue from a mouse that had been dosed orally (90 mg/kg) with R,S-sulforaphane 3 h prior to sacrifice. Sulforaphane and its glutathione and N-acetyl cysteine conjugates were monitored with selected reaction monitoring and detected in the stomach and various other tissues from the dosed mouse. No signal for these species was observed in the tissue from a control mouse. The same dosed-tissue section was used to illustrate the possibility of obtaining a lane scan across the whole-body section. In total, these results illustrate the potential for rapid screening of the distribution of drugs and metabolites in thin tissue sections with the liquid micro-junction surface sampling probe/electrospray mass spectrometry approach. Published in 2007 by John Wiley & Sons, Ltd.
机译：一种自吸式的液体微型表面采样探针/电喷雾发射器质谱。证明了系统用于直接分析发现和给药的药物及其在薄组织切片中的代谢物。首先通过栅格扫描在肝脏部分的叶片上的一部分大鼠肝脏的区域上首先通过组织进行原则上的验证和分析而不需要样品制备。来自所选反应监测的质谱信号用于在组织上开发斑点药物的化学图像。探针还用于在牺牲之前从口服（90mg / kg）的小鼠中选择性地点发现矢状全身组织的样品区域，在牺牲之前用R，S-磺酰丙烷3小时。用选定的反应监测监测亚磺素及其谷胱甘肽和N-乙酰半胱氨酸缀合物，并在胃中检测到来自胃部的各种其他组织。从对照小鼠的组织中没有观察到这些物种的信号。使用相同的剂量组织部分来说明在整个体部分中获得车道扫描的可能性。总的来说，这些结果说明了利用液体微结表面采样探针/电喷雾质谱法在薄膜切片中快速筛选药物和代谢物分布的可能性。在2007年由John Wiley＆Sons，Ltd。发表于2007年

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