• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of mass spectrometry: JMS >Chip‐based high resolution tandem mass spectrometric determination of fibroblast growth factor—chondroitin sulfate disaccharides noncovalent interaction
【24h】

Chip‐based high resolution tandem mass spectrometric determination of fibroblast growth factor—chondroitin sulfate disaccharides noncovalent interaction

机译：基于芯片的高分辨率串联质谱法测定成纤维细胞生长因子 - 软骨素硫酸盐二糖的非共价相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract >Fibroblast growth factor‐2 (FGF‐2) is involved in wound healing and embryonic development. Glycosaminoglycans (GAGs), the major components of the extracellular matrix (ECM), play fundamental roles at this level. FGF‐GAG noncovalent interactions are in the focus of research, due to their influence upon cell proliferation and tissue regeneration. Lately, high resolution mass spectrometry (MS) coupled with chip‐nanoelectrospray (nanoESI) contributed a significant progress in glycosaminoglycomics by discoveries related to novel species and their characterization. We have employed a fully automated chip‐nanoESI coupled to a quadrupole time‐of‐flight (QTOF) MS for assessing FGF‐GAG noncovalent complexes. For the first time, a CS disaccharide was involved in a binding assay with FGF‐2. The experiments were conducted in 10?mM ammonium acetate/formic acid, pH?6.8, by incubating FGF‐2 and CS in buffer. The detected complexes were characterized by top‐down in tandem MS (MS/MS) using collision induced‐dissociation (CID). CID MS/MS provided data showing for the first time that the binding process occurs via the sulfate group located at C4 in GalNAc. This study has demonstrated that chip‐MS may generate reliable data upon the formation of GAG‐protein complexes and their structure. Biologically, the findings are relevant for studies focused on the identification of the active domains in longer GAG chains. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >成分增长因子-2（FGF-2）参与伤口愈合和胚胎发育。糖胺聚糖（GAG），细胞外基质（ECM）的主要成分，在这个水平上起着基本角色。由于它们对细胞增殖和组织再生的影响，FGF-GAG非共价相互作用处于研究焦点。最近，与芯片纳米电池粥样花（Nanooinei）偶联的高分辨率质谱（MS）通过与新种类及其表征相关的发现导致糖胺蛋白酶术中的显着进展。我们采用了一种全自动芯片纳米型，耦合到四极杆飞行时间（QTOF）MS，用于评估FGF-GAG非共价复合物。首次，Cs二糖涉及FGF-2的结合测定。通过在缓冲液中孵育FGF-2和Cs，在10·mm乙酸铵/甲酸，pHα66中进行实验。通过碰撞诱导 - 解离（CID），通过串联MS（MS / MS）自上而下来表征检测到的复合物。 CID MS / MS提供了第一次通过位于Galnac的C4的硫酸盐基团发生结合过程的数据。该研究表明，芯片MS可以在形成GAG蛋白复合物及其结构时产生可靠的数据。生物学上，研究结果与重点关注在较长的GAG链中鉴定活性结构域的研究。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS》</a> <b style="margin: 0 2px;">|</b><span>2018年第7期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Robu Adrian C.&option=202" target="_blank" rel="nofollow">Robu Adrian C.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Popescu Laurentiu&option=202" target="_blank" rel="nofollow">Popescu Laurentiu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Seidler Daniela G.&option=202" target="_blank" rel="nofollow">Seidler Daniela G.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zamfir Alina D.&option=202" target="_blank" rel="nofollow">Zamfir Alina D.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Mass Spectrometry LaboratoryNational Institute for Research and Development in Electrochemistry and Condensed MatterPlautius Andronescu Str. 1 RO‐300224 Timisoara Romania;</p> <p>Mass Spectrometry LaboratoryNational Institute for Research and Development in Electrochemistry and Condensed MatterPlautius Andronescu Str. 1 RO‐300224 Timisoara Romania;</p> <p>Department of Gastroentero‐ Hepato‐ and Endocrinology I3Hannover Medical SchoolEB2/R3110 Carl‐Neuberg‐Str. 1 D‐30625 Hannover Germany;</p> <p>Mass Spectrometry LaboratoryNational Institute for Research and Development in Electrochemistry and Condensed MatterPlautius Andronescu Str. 1 RO‐300224 Timisoara Romania;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chip‐nanoelectrospray&option=203" rel="nofollow">chip‐nanoelectrospray;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fibroblast growth factor‐2&option=203" rel="nofollow">fibroblast growth factor‐2;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=glycosaminoglycans&option=203" rel="nofollow">glycosaminoglycans;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high resolution mass spectrometry&option=203" rel="nofollow">high resolution mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=noncovalent interaction&option=203" rel="nofollow">noncovalent interaction;</a> </p> <div class="translation"> 机译：芯片纳米电酶涂布;成纤维细胞生长因子-2;糖胺聚糖;高分辨率质谱;非共价互动; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025633331.html">Chip‐based high resolution tandem mass spectrometric determination of fibroblast growth factor—chondroitin sulfate disaccharides noncovalent interaction</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Robu Adrian C.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Robu Adrian C.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Popescu Laurentiu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Popescu Laurentiu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Seidler Daniela G.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Seidler Daniela G.,</a> <a href="/journal-foreign-34906/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of mass spectrometry: JMS .</a> <span>2018</span><span>,第7期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于芯片的高分辨率串联质谱法测定成纤维细胞生长因子 - 软骨素硫酸盐二糖的非共价相互作用</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070402682299.html">Interactions That Influence the Binding of Synthetic Heparan Sulfate Based Disaccharides to Fibroblast Growth Factor?2</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yi-Ching Li&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yi-Ching Li,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=I-Hsin Ho&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">I-Hsin Ho,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chiao-Chu Ku&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chiao-Chu Ku,</a> <a href="/journal-foreign-18812/" target="_blank" rel="nofollow" class="tuijian_authcolor">ACS Chemical Biology .</a> <span>2014</span><span>,第8期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：影响合成硫酸乙酰肝素基二糖与成纤维细胞生长因子？2结合的相互作用。</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_journal-the-american-chemical-society_thesis/0204111828184.html">Divergent Synthesis of 48 Heparan Sulfate-Based Disaccharides and Probing the Specific Sugar-Fibroblast Growth Factor-1 Interaction</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yu-Peng Hu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yu-Peng Hu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yong-Qing Zhong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yong-Qing Zhong,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhi-Geng Chen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhi-Geng Chen,</a> <a href="/journal-foreign-719/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of the American Chemical Society .</a> <span>2012</span><span>,第51期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：48种硫酸乙酰肝素双糖的不同合成和探索特定的糖-成纤维细胞生长因子-1相互作用。</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-256173_thesis/0705010088713.html">Fully automated chip-based nanoelectrospray tandem mass spectrometry for the determination of noncovalent protein-carbohydrate interaction</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alina D. Zamfir&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Alina D. Zamfir,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Martin G. Peter&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Martin G. Peter,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jasna Peter-Katalinic&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jasna Peter-Katalinic </a> <a href="/conference-foreign-256173/" target="_blank" rel="nofollow" class="tuijian_authcolor">ASMS Conference on Mass Spectrometry and Allied Topics .</a> <span>2006</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于自动化的芯片的纳米电池串联质谱法测定非共价蛋白 - 碳水化合物相互作用</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061603636.html">Distribution of Bone Morphogenetic Proteins and BMP receptors in Osteochondromas and Modulation of Transforming Growth Factor-beta1 Actions by Heparan Sulfate and Chondroitin Sulfate in Normal Articular Cartilage.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cuellar, Araceli.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Cuellar, Araceli. </a> <span>2014</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：骨软骨瘤中骨形态发生蛋白和BMP受体的分布以及正常软骨中硫酸乙酰肝素和硫酸软骨素的转化生长因子-β1行为的调节。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040004489631.html">A tandem mass spectrometric approach to determination of chondroitin/dermatan sulfate oligosaccharide glycoforms</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=May Joy C. Miller&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">May Joy C. Miller,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Catherine E. Costello&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Catherine E. Costello,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Anders Malmström&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Anders Malmström,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：串联质谱法测定软骨素/硫酸皮肤素寡糖糖型</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100048346862.html">Tandem mass spectrometric strategies for determination of sulfation positions and uronic acid epimerization in chondroitin sulfate oligosaccharides</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zaia Joseph&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zaia Joseph,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Xue-Qing&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Li Xue-Qing,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chan Shiu-Yung&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chan Shiu-Yung,</a> <span>2003</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：串联质谱法测定硫酸软骨素寡糖中的硫酸化位置和糖醛酸差向异构。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-light-industry_thesis/0201291809496.html">基于动态共价键和非共价键相互作用的自愈合水凝胶研究进展</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘瑞雪&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘瑞雪</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈纪超&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈纪超</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李迎博&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李迎博</a> <span> <a href="/journal-cn-23780/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 轻工学报 </a> </span> <span> . 2021</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_modern-food_thesis/0201289498719.html">单宁酸-鱼明胶共价、非共价相互作用复合物抗氧化活性比较研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=郭扬凯&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 郭扬凯</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=赵利&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,赵利</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=白春清&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,白春清</a> <span> <a href="/journal-cn-12460/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 现代食品 </a> </span> <span> . 2021</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_grain-distribution-technology_thesis/0201289475966.html">单宁酸-鱼明胶共价、非共价相互作用复合物抗氧化活性比较研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=郭扬凯&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 郭扬凯</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=赵利&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,赵利</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=白春清&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,白春清</a> <span> <a href="/journal-cn-9364/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 粮食流通技术 </a> </span> <span> . 2021</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_academic-news-chengdu_thesis/0201286728134.html">基于高阶密度泛函方法研究联吡啶类有机金属配合物的电子结构及非共价相互作用</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王辉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王辉</a> <span> <a href="/journal-cn-9993/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 学术动态（成都） </a> </span> <span> . 2014</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_china-food-additives_thesis/020125118380.html">液相色谱串联质谱法测定食品中甘露糖醇、麦芽糖、木糖醇、山梨糖醇</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=马书民&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 马书民</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王明泰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王明泰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=韩大川&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,韩大川</a> <span> <a href="/journal-cn-9438/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国食品添加剂 </a> </span> <span> . 2011</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-60898_thesis/020222686013.html">可控自组装体系中的新型非共价和动态共价相互作用研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王梅祥&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王梅祥</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐昕&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐昕</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王德先&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王德先</a> <span> <a href="/conference-cn-60898/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . “可控自组装体系及其功能化”重大研究计划2015-2016年度学术交流会 </a> <span> <span> . 2016</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020313560172.html">基于动态共价键、非共价相互作用构筑响应型乳液的研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=任改焕&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 任改焕</a> <span> . 2019</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120103974741.html">一种快速亲水相互作用色谱-串联质谱法测定血浆中二甲双胍的方法</a> <b>[P]</b> . <span> 中国专利： CN111413439A </span> <span> . 2020-07-14</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061204847175.html">基于碘量法测定过氧一硫酸盐、过氧二硫酸盐和过氧化氢混合溶液中各过氧化物浓度的方法</a> <b>[P]</b> . <span> 中国专利： CN110632250B </span> <span> . 2021.12.21</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130455075057.html">Preservation solution for ex vivo, warm preservation of tissues, explants, organs and vascular endothelial cells comprising retinal- derived fibroblast growth factor, cyclodextrin and chondroitin sulfate</a> <b>[P]</b> . <span> 外国专利： <!-- 美国专利： --> US5599659A </span> <span> . 1997-02-04</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于离体，温热保存组织，外植体，器官和血管内皮细胞的保存液，包含视网膜衍生的成纤维细胞生长因子，环糊精和硫酸软骨素 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130433280256.html">Analysis of insulin-like growth factors from biological fluids by the use of affinity-based mass spectrometric methods</a> <b>[P]</b> . <span> 外国专利： <!-- 美国专利： --> US2008064044A9 </span> <span> . 2008-03-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用基于亲和力的质谱法分析生物液体中的胰岛素样生长因子 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130440326093.html">Analysis of insulin-like growth factors from biological fluids by the use of affinity-based mass spectrometric methods</a> <b>[P]</b> . <span> 外国专利： <!-- 美国专利： --> US2005032116A1 </span> <span> . 2005-02-10</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用基于亲和力的质谱法分析生物液体中的胰岛素样生长因子 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025633331','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche"> <!-- <a href="javascript:void(0);" onclick="link_analysis('/shoppingcart/auth/list.html',this)">购物车</a>--> </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704025633331down" data-source="7," data-out-id="upzAU1HCY3Tk/0o24rMzyw==," data-f-source-id="7" data-title="Chip‐based high resolution tandem mass spectrometric determination of fibroblast growth factor—chondroitin sulfate disaccharides noncovalent interaction" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="34906" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Chip‐based high resolution tandem mass spectrometric determination of fibroblast growth factor—chondroitin sulfate disaccharides noncovalent interaction"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.7"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.7"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>