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首页> 外文期刊>Biotechnology Progress >Numerical Evaluation of Lactoperoxidase Inactivation During Continuous Pulsed Electric Field Processing
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Numerical Evaluation of Lactoperoxidase Inactivation During Continuous Pulsed Electric Field Processing

机译：连续脉冲电场处理过程中乳过氧化物酶失活的数值评估

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摘要

A computational fluid dynamics (CFD) model describing the flow, electric field and temperature distribution of a laboratory-scale pulsed electric field (PEF) treatment chamber with co-field electrode configuration was developed. The predicted temperature increase was validated by means of integral temperature studies using thermocouples at the outlet of each flow cell for grape juice and salt solutions. Simulations of PEF treatments revealed intensity peaks of the electric field and laminar flow conditions in the treatment chamber causing local temperature hot spots near the chamber walls. Furthermore, thermal inactivation kinetics of lactoperoxidase (LPO) dissolved in simulated milk ultrafiltrate were determined with a glass capillary method at temperatures ranging from 65 to 80°C. Temperature dependence of first order inactivation rate constants was accurately described by the Arrhenius equation yielding an activation energy of 597.1 kJ mol~(-1) . The thermal impact of different PEF processes on LPO activity was estimated by coupling the derived Arrhenius model with the CFD model and the predicted enzyme inactivation was compared to experimental measurements. Results indicated that LPO inactivation during combined PEFIthermal treatments was largely due to thermal effects, but 5-12% enzyme inactivation may be related to other electro-chemical effects occurring during PEF treatments.
机译：建立了计算流体动力学（CFD）模型，该模型描述了带有同场电极配置的实验室规模脉冲电场（PEF）处理室的流量，电场和温度分布。通过在每个流通池出口处使用热电偶对葡萄汁和盐溶液进行积分温度研究，验证了预测的温度升高。 PEF处理的模拟显示了处理室内电场强度和层流条件的强度峰值，从而在处理室壁附近引起局部温度热点。此外，使用玻璃毛细管法在65至80°C的温度下测定了溶解在模拟超滤液牛奶中的乳过氧化物酶（LPO）的热失活动力学。一阶失活速率常数的温度依赖性由Arrhenius方程精确描述，产生的活化能为597.1 kJ mol〜（-1）。通过将派生的Arrhenius模型与CFD模型耦合来估计不同PEF工艺对LPO活性的热影响，并将预测的酶失活与实验测量值进行比较。结果表明，在联合PEFI热处理过程中LPO失活主要是由于热效应，但是5-12％的酶失活可能与在PEF治疗过程中发生的其他电化学效应有关。

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