• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Nisin Production of Lactococcus lactis N8 with Hemin-Stimulated Cell Respiration in Fed-Batch Fermentation System
【24h】

Nisin Production of Lactococcus lactis N8 with Hemin-Stimulated Cell Respiration in Fed-Batch Fermentation System

机译：补料分批发酵系统中由血红素刺激的细胞呼吸作用产生乳酸乳球菌N8的乳链菌肽

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, nisin production of Lactococcus lactis N8 was optimized by independent variables of glucose, hemin and oxygen concentrations in fed-batch fermentation in which respiration of cells was stimulated with hemin. Response surface model was able to explain the changes of the nisin production of L. lactis N8 in fed-batch fermentation system with high fidelity (R-2 98%) and insignificant lack of fit. Accordingly, the equation developed indicated the optimum parameters for glucose, hemin, and dissolved oxygen were 8 g L-1 h(-1), 3 mu g mL(-1) and 40%, respectively. While 1711 IU mL(-1) nisin was produced by L. lactis N8 in control fed-batch fermentation, 5410 IU mL(-1) nisin production was achieved within the relevant optimum parameters where the respiration of cell was stimulated with hemin. Accordingly, nisin production was enhanced 3.1 fold in fed-batch fermentation using hemin. In conclusion the nisin production of L. lactis N8 was enhanced extensively as a result of increasing the biomass by stimulating the cell respiration with adding the hemin in the fed-batch fermentation. (C) 2015 American Institute of Chemical Engineers
机译：在这项研究中，乳酸，乳酸乳球菌N8的乳酸链球菌素的生产是通过补料分批发酵中葡萄糖，血红素和氧气浓度的独立变量来优化的，在分批发酵中，用血红素刺激细胞的呼吸。响应面模型能够解释高保真度（R-2 98％）和缺乏适度缺乏的补料分批发酵系统中乳酸乳球菌N8乳酸链球菌素产生的变化。因此，开发的方程表明，葡萄糖，血红素和溶解氧的最佳参数分别为8 g L-1 h（-1），3μgmL（-1）和40％。虽然乳酸乳球菌N8在补料分批发酵中产生了1711 IU mL（-1）乳酸链球菌素，但在相关的最佳参数范围内却获得了5410 IU mL（-1）乳酸链球菌素的产生，其中用血红素刺激细胞的呼吸。因此，使用血红素在补料分批发酵中乳链菌肽的产量提高了3.1倍。总之，由于通过在补料分批发酵中添加血红素来刺激细胞呼吸，从而增加了生物量，乳酸乳球菌N8的乳酸链球菌素生产得到了广泛提高。 （C）2015美国化学工程师学会

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号