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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Enhanced bone regeneration and visual monitoring via superparamagnetic iron oxide nanoparticle scaffold in rats
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Enhanced bone regeneration and visual monitoring via superparamagnetic iron oxide nanoparticle scaffold in rats

机译：通过超顺磁性氧化铁纳米粒子支架在大鼠中增强骨再生和视觉监测

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A main challenge for use of scaffolds in bone engineering involves non-invasive monitoring in vivo and enhanced bone regeneration. The tissue repair effect of superparamagnetic iron oxide nanoparticles (SPIONs) was demonstrated previously by our group. However, testing in vivo is needed to confirm in vitro results. Here, SPIONs loaded gelatin sponge (GS) was used as a scaffold (SPIONs-GS) and implanted in the incisor sockets of Sprague-Dawley rats. Incisor sockets filled with nothing and filled with GS served as controls. Rats were sacrificed at 2 and 4weeks. A significant decrease in the signal intensity of T2-weighted magnetic resonance imaging (MRI) in the SPIONs-GS group was noted. Changes in image intensity of scaffolds (indicating scaffold degradation and interaction with host tissues) could be visually monitored over time. Microcomputed tomography showed that the SPIONs-GS group had more newly formed bone (64.44 +/- 10.92 vs. 28.1 +/- 4.49, p .0001) and a better preserved alveolar ridge than blank control group at 4 weeks (0.962 +/- 0.01 vs. 0.92 +/- 0.01, p .0001). Histology confirmed imaging results, showing good consistency in new bone formation and scaffold degradation. The number of SPIONs decreased rapidly with time due to quick degradation of GS, whereas the number of endocytic SPIONs in cells increased with time. These residual SPIONs, together with newly formed bone, could be detected by MRI at 4weeks. Therefore, it was clear that SPIONs induced active osteogenesis. In conclusion, good visibility on MRI and enhanced regeneration of bone can be obtained by implanting SPIONs-GS in vivo without using an external magnetic field.
机译：在骨骼工程中使用支架的主要挑战涉及体内无侵入性监测和增强的骨再生。通过我们的基团证明了超顺磁性氧化铁纳米粒子（胶片）的组织修复效果。然而，需要在体内进行测试以确认体外结果。这里，散热的明胶海绵（GS）用作支架（散氏酱）并植入Sprague-Dawley大鼠的切牙插座中。切牙套接字填充没有，并充满了GS作为控制。在2和4周中处死大鼠。注意到散斑-GS组中T2加权磁共振成像（MRI）的信号强度的显着降低。随着时间的推移，可以在视觉上监测支架的图像强度的变化（表明支架降解和与宿主组织的相互作用）。微仿性断层扫描表明，酱GS组具有更新形成的骨（64.44 +/- 10.92与28.1 +/- 4.49，P＆ .0001），并且在4周（0.962 + / - 0.01 vs.0.92 +/- 0.01，P＆ 0001）。组织学证实了成像结果，显示出新的骨形成和支架降解的良好一致性。由于GS的快速降解，沟数随时间随时间迅速下降，而细胞中的内吞氏料的数量随时间而增加。这些残留的酱与新形成的骨骼一起可以通过MRI在4周上检测到。因此，显然酱源性诱导有活性骨质发生。总之，通过在不使用外部磁场的情况下植入体内的散杆-GS，可以获得对MRI和增强的骨再生的良好能见度。

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