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Occurrence of ochratoxin A in animal tissues and feeds in Poland in 2014-2016

机译:2014 - 2016年波兰动物组织中Ochratoxin A的发生

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Introduction: Ochratoxin A (OTA) is a toxic metabolite mainly produced by Aspergillus spp. and Penicillum spp. fungi. Research on the contamination of cereals, complete feeds, and tissues with this mycotoxin has indicated that it can be a toxicological problem impacting animal health and food safety in temperate climes. OTA contamination mainly besets the global pig industry, necessitating the monitoring of feeds and animal tissues. The aim of the study was to present the results of the official monitoring of OTA in animal tissues and feeds in Poland in 2014-2016 and determine the possible correlation between the presence of OTA in different types of samples. Material and Methods: The presence of ochratoxin A was determined using accepted procedures based on liquid chromatography with fluorescence detection after immunoaffinity column clean-up. Determination of OTA was afforded in the range of 0.3 mu g/kg to 300 mu g/kg in complete feeds and from 0.2 mu g/kg to 150 mu g/kg in the kidneys, liver, and muscles. Results: Over the three year span, about 23.5% of the animal tissues samples were contaminated by ochratoxin A. In the 2014 survey, 10% of the sample tissues contained 5-10 mu g/kg (only one sample above 10 mu g/kg), and in 2015 and 2016, 24% of samples showed levels above the limit of quantification 0.2 mu g/kg, while none of the samples exceeded the established provisional action level of 5 mu g/kg for animal tissues. The animal feed analysis showed that 9% was contaminated with ochratoxin A above the limit of quantification of 0.3 mu g/kg. In 2% of feed samples the OTA concentration was greater than 50 mu g/kg. Conclusion: The results confirm the appropriacy of OTA contamination monitoring and help to increase food safety.
机译:介绍:Ochratoxina(ota)是主要由Aspergillus SPP产生的有毒代谢物。和青霉素spp。菌类。谷物,完整饲料和组织污染的研究表明,它可以是毒理学问题影响动物健康和食品安全在温带群体中。 OTA污染主要围攻全球猪工业,需要监测饲料和动物组织。该研究的目的是展示2014 - 2016年在波兰的动物组织中OTA官方监测结果,并确定不同类型样品中OTA之间的可能相关性。材料和方法:使用基于液相色谱法测定Ochratoxin A的存在,在免疫亲和柱清洗后荧光检测。在完全饲料中的0.3μg/ kg至300μg/ kg的范围内,在肾脏,肝脏和肌肉中的0.3μg/ kg至300μg/ kg的范围内得到0.3μg/ kg至150μg/ kg。结果:三年跨度,约23.5%的动物组织样品被Ochratoxin A污染。在2014年调查中,10%的样品组织含有5-10μg/ kg(仅在10 mu g以上的一个样品。 KG),在2015年和2016年,24%的样品显示出高于定量的水平0.2μg/ kg,而这些样品中没有任何样品超过动物组织的5μg/ kg的临时作用水平。动物饲料分析表明,9%的污染了高于0.3μg/ kg的量化极限的ochratoxin a。在2%的进料样品中,OTA浓度大于50μg/ kg。结论:结果证实了OTA污染监测的适当占用,有助于提高食品安全。

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