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首页> 外文期刊>Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics >Molecular dissection of egg fertilization signaling with the aid of tyrosine kinase-specific inhibitor and activator strategies
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Molecular dissection of egg fertilization signaling with the aid of tyrosine kinase-specific inhibitor and activator strategies

机译：借助酪氨酸激酶特异性抑制剂和激活剂策略对卵子受精信号进行分子解剖

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摘要

Fertilization is triggered by sperm–egg interaction and fusion that initiate a transient rise(s) in the free intracellular calcium ([Ca~(2+)]_i) that is responsible for a series of biochemical and cell biological events, so-called "egg activation". Calcium-dependent egg activation leads to the initiation of developmental program that culminates in the birth of individuals. A growing body of knowledge has uncovered the molecular mechanisms underlying sperm-induced transient [Ca~(2+)]_i increase(s) to some extent; namely, in most animals so far studied, a second messenger inositol 1,4,5-trisphosphate (IP_3) seems to play a pivotal role in inducing [Ca~(2+)]_i transient(s) at fertilization. However, signaling mechanisms used by sperm to initiate IP_3-[Ca~(2+)]_i transient pathway have not been elucidated. To approach this problem, we have employed African clawed frog, Xenopus laevis, as a model animal and conducted experiments designed specifically to determine the role of the Src family protein-tyrosine kinases (SFKs or Src family PTKs) in the sperm-induced egg activation. This review compiles information about the use of PTK-specific inhibitors and activators for analyzing signal transduction events in egg fertilization. Specifically, we focus on molecular identification of Xenopus Src and the signaling mechanism of the Src-dependent egg activation that has been established recently. We also summarize recent advances in understanding the role of the Src family kinases in egg fertilization of other model organisms, and discuss future directions of the field.
机译：受精是由精子与卵的相互作用和融合引起的，这些相互作用引发了细胞内游离钙（[Ca〜（2 +）] _ i）的短暂升高，从而引起一系列生化和细胞生物学事件，即所谓的“蛋激活”。钙依赖性卵子活化导致发育程序的启动，最终导致个体的出生。越来越多的知识揭示了精子诱导的瞬时[Ca〜（2 +）] _ i增加的分子机制。即，在迄今为止研究的大多数动物中，第二种信使肌醇1,4,5-三磷酸酯（IP_3）似乎在受精时诱导[Ca〜（2 +）] _ i瞬变中起关键作用。然而，尚未阐明精子用来启动IP_3- [Ca〜（2 +）] _ i瞬时途径的信号传导机制。为了解决这个问题，我们以非洲爪蛙Xenopus laevis为模型动物，并进行了专门设计的实验，以确定Src家族蛋白酪氨酸激酶（SFK或Src家族PTK）在精子诱导的卵子活化中的作用。 。这篇综述汇编了有关使用PTK特异性抑制剂和活化剂来分析卵子受精过程中信号转导事件的信息。具体来说，我们专注于非洲爪蟾Src的分子鉴定和最近建立的Src依赖性卵细胞活化的信号传导机制。我们还总结了了解Src家族激酶在其他模式生物的卵受精中的作用的最新进展，并讨论了该领域的未来方向。

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