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首页> 外文期刊>American journal of rhinology >Fungal allergens induce cathelicidin LL-37 expression in chronic rhinosinusitis patients in a nasal explant model.
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Fungal allergens induce cathelicidin LL-37 expression in chronic rhinosinusitis patients in a nasal explant model.

机译：真菌过敏原在鼻外植体模型的慢性鼻-鼻窦炎患者中诱导cathelicidin LL-37表达。

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BACKGROUND: Fungus is thought to play an important role in some subgroups of chronic rhinosinusitis (CRS) patients with eosinophilic mucus (EMCRS). The cathelicidin LL-37 is an important innate defense peptide with antimicrobial activity but its responses in CRS and EMCRS patients have not been established. We investigated the innate immune responses of LL-37 in nasal tissue from CRS and EMCRS patients to fungal allergen challenge. METHODS: The levels of LL-37 produced by nasal tissue and secreted in response to fungal allergen challenge were determined by a nasal tissue explant in vitro model. LL-37 mRNA and protein levels were quantified by real-time reverse-transcriptase-polymerase chain reaction and immunoassay methods. RESULTS: LL-37 mRNA expression in CRS, but not EMCRS patients, is significantly upregulated by Aspergillus (mean fourfold increase) and Alternaria (mean sixfold increase) extracts in a dose-response manner (p < 0.001). LL-37 peptide levels in the nasal tissue from CRS patients are increased in response to Alternaria (p < 0.05). In contrast, with EMCRS patients, the expression of LL-37 peptide in nasal tissue is increased with Aspergillus (p < 0.001) but is reduced with Alternaria. We also observed a trend where levels of secreted LL-37 were decreased with higher doses of Alternaria and Aspergillus extracts. CONCLUSION: LL-37 is significantly up-regulated at the mRNA and protein level in CRS patients in response to fungal allergens. However, EMCRS patients do not show increased LL-37 at either the mRNA or the protein level in response to Alternaria.
机译：背景：真菌被认为在慢性鼻鼻窦炎（CRS）嗜酸性粘液（EMCRS）患者的某些亚组中起着重要作用。 cathelicidin LL-37是具有抗菌活性的重要先天防御肽，但尚未确定其对CRS和EMCRS患者的反应。我们调查了CRS和EMCRS患者鼻腔组织中LL-37对真菌过敏原激发的先天免疫反应。方法：通过鼻组织外植体体外模型测定鼻组织产生并响应真菌过敏原攻击而分泌的LL-37水平。通过实时逆转录酶-聚合酶链反应和免疫测定方法定量LL-37 mRNA和蛋白水平。结果：曲霉提取物（平均增加4倍）和链霉菌（平均增加6倍）提取物以剂量反应的方式显着上调了CRS中但非EMCRS患者中的LL-37 mRNA表达（p <0.001）。 CRS患者的鼻腔组织中的LL-37肽水平响应链格孢菌而增加（p <0.05）。相比之下，对于EMCRS患者，曲霉菌会增加鼻腔组织中LL-37肽的表达（p <0.001），而链格孢菌会降低。我们还观察到了趋势，即随着剂量的链霉菌和曲霉提取物的使用，分泌的LL-37的水平降低。结论：CRS患者响应真菌过敏原时，LL-37在mRNA和蛋白质水平上显着上调。但是，EMRS病人对交链孢菌的反应无论在mRNA水平还是蛋白水平均未显示LL-37升高。

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