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首页> 外文期刊>Current Biology: CB >A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation
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A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation

机译：Kinesin-5，Cin8，促进蛋白磷酸酶1至Kinetochores并调节染色体隔离

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摘要

Kinesin-5 is a highly conserved homo-tetrameric protein complex responsible for crosslinking microtubules and pushing spindle poles apart. The budding yeast Kinesin-5, Cin8, is highly concentrated at kinetochores in mitosis before anaphase, but its functions there are largely unsolved. Here, we show that Cin8 localizes to kinetochores in a cell-cycle-dependent manner and concentrates near the microtubule binding domains of Ndc80 at metaphase. Cin8’s kinetochore localization depends on the Ndc80 complex, kinetochore microtubules, and the Dam1 complex. Consistent with its kinetochore localization, a Cin8 deletion induces a loss of tension at the Ndc80 microtubule binding domains and a major delay in mitotic progression. Cin8 associates with Protein Phosphatase 1 (PP1), and mutants that inhibit its PP1 binding also induce a loss of tension at the Ndc80 microtubule binding domains and delay mitotic progression. Taken together, our results suggest that Cin8-PP1 plays a critical role at kinetochores to promote accurate chromosome segregation by controlling Ndc80 attachment to microtubules.
机译：Kinesin-5是一种高度保守的同性全角蛋白质复合体，其负责交联微管和推动轴杆间隔。萌芽的酵母Kinesin-5，CIN8，在后期的有丝分裂中高度浓缩，但其功能在很大程度上是未解决的。在这里，我们表明CIN8以细胞周期依赖性方式定位于Kinetochores，并在中期的NDC80的微管结合结构域附近浓缩。 CIN8的Kinetochore本地化取决于NDC80复合体，Kinetochore Microtubules和Dam1复合物。 CIN8缺失符合其KINETOCHORE定位，CIN8缺失在NDC80微管结合结构域的张力丧失和有丝分裂进展的主要延迟。 CIN8与蛋白质磷酸酶1（pp1）相关联，抑制其PP1结合的突变体也诱导NDC80微管结合结构域的张力损失和延迟有丝分裂进展。我们的结果表明，CIN8-PP1在Kinetochores在Kinetochores上发挥着关键作用，以通过控制NDC80附着于微管来促进精确的染色体隔离。

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