...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology and Biotechnology >New two-component regulatory system required for the constitutive expression of bph operon in Cupriavidus basilensis WS
【24h】

New two-component regulatory system required for the constitutive expression of bph operon in Cupriavidus basilensis WS

机译：在Cupriavidus Basilensis WS中BPH操纵子本构表达所需的新双组分监管系统

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cupriavidus basilensis WS degrades diphenyl ether (DE) and its lower brominated derivatives using enzymes encoded by the bph operon. However, it is not yet known under what circumstances bph genes are expressed and how they are regulated in C. basilensis WS. To answer these questions, we used transposon mutagenesis and identified a new two-component regulatory system, BphS/BphT, in C. basilensis WS, which is indispensable for the expression of the bph operon. When BphS or BphT is inactivated, C. basilensis WS no longer exhibits the ability to decompose DE. Using a -galactosidase reporter system and RT-qPCR, we showed that bph genes are constitutively transcribed in C. basilensis WS and that deletion of bphS or bphT strongly inhibited the transcription of bph genes. We also showed that the gene ORF0, which is upstream of bphA1 and is similar to the GntR-family regulators of the bph operon, is not involved in the constitutive transcription of the bph operon in C. basilensis WS. The cis-acting elements required for the expression and regulation of bph genes in the DE degradation pathway are included in the intergenic region between ORF0 and bphA1. Our results suggest that BphS/BphT represents a new two-component regulatory system for the bph operon that is necessary for the constitutive expression of bph genes.
机译：Cupriavidus Basilensis WS使用BPH操纵子编码的酶降解二苯基醚（DE）及其低溴化衍生物。然而，在表达BPH基因的情况下尚不清楚，并且它们如何在C. basilensis WS中调节。为了回答这些问题，我们使用转座子诱变，并确定了一种新的双组分调节系统，BPHS / BPHT，在C. basilensis Ws中，这对于BPH操纵子的表达是必不可少的。当BPHS或BPHT灭活时，C.Basilensis Ws不再表现出解析de的能力。使用 - 高碳糖苷酶报告系统和RT-QPCR，我们表明BPH基因在C.益杆菌WS中组成型转录，并且缺失BPHS或BPHT强烈抑制BPH基因的转录。我们还表明，在BPHA1上游的基因ORF0并类似于BPH型术的GNTR家族调节因子，不参与C. BPHOSINIS WS中BPH操纵子的组成型转录。在DE降解途径中表达和调节BPH基因所需的顺式作用元件包括在ORF0和BPHA1之间的代际区域中。我们的研究结果表明，BPHS / BPHT代表了BPH型BPH运动序的新的双组分调节系统，这对于BPH基因的组成型表达所必需的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号