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首页> 外文期刊>Applied Microbiology and Biotechnology >Beneficial effects of Enterococcus faecalis in hypercholesterolemic mice on cholesterol transportation and gut microbiota
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Beneficial effects of Enterococcus faecalis in hypercholesterolemic mice on cholesterol transportation and gut microbiota

机译：肠球菌粪便在胆固醇运输和肠道微生物对高胆固醇小鼠中的有益效果

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Hypercholesterolemia plays a critical role in the development of atherosclerosis and cardiovascular diseases. Many works have been reported that gut microbiota could affect hypercholesterolemia through cholesterol metabolism. However, the role of gut microbiota on cholesterol transportation remains unclear. In this study, 8-week-old C57BL/6J mice were fed with high-cholesterol diet to build the hypercholesterolemic mice. Then, the hypercholesterolemic mice got the oral administration of Enterococcus faecalis ATCC19433 at a dose of 10(9)CFU/mL/day or PBS with high-cholesterol diet for 4weeks. Serum was collected to detect the concentration of total cholesterol (TC). Meanwhile, pathology, histology, real-time polymerase chain reaction, Western blot, and immunofluorescence were used to evaluate the expression of ABCG5 and ABCG8 in the liver and small intestine. We also analyzed the composition of gut microbiota through high-throughput sequencing method. Oral administration of E. faecalis ATCC19433 significantly decreased the concentration of serum cholesterol in hypercholesterolemic mice. Furthermore, E. faecalis ATCC19433 reduced the concentration of liver cholesterol and improved cholesterol by increasing the expression of ABCG5 and ABCG8. Moreover, oral administration of E. faecalis ATCC19433 modulated the composition of gut microbiota and increased the counts of Lactobacillus, Bifidobacterium, and Akkermansia. Our results showed that E. faecalis ATCC19433 could exert hypocholesterolemic effect on hypercholesterolemic mice by improving transporter ABCG5 and ABCG8. E. faecalis ATCC19433 maybe contribute to the transportation of cholesterol potentially and modulate the composition of gut microbiota.
机译：高胆固醇血症在动脉粥样硬化和心血管疾病的发展中起着关键作用。据报道，许多作品肠道微生物会通过胆固醇新陈代谢来影响高胆固醇血症。然而，肠道微生物酵母对胆固醇运输的作用仍然尚不清楚。在这项研究中，将8周龄C57BL / 6J小鼠饲喂高胆固醇饮食以构建高胆固醇小鼠。然后，高胆固醇小鼠以10（9）个CFU / ml /天或PBS的剂量为4周的剂量，以10（9）CFU / ml /天或PBS的剂量进行口服给予肠球菌。收集血清以检测总胆固醇（TC）的浓度。同时，使用病理学，组织学，实时聚合酶链反应，蛋白质印迹和免疫荧光来评估ABCG5和ABCG8在肝脏和小肠中的表达。我们还通过高通量测序方法分析了肠道微生物酵母的组成。 E.Acecalis ATCC19433的口服给药显着降低了高胆固醇小鼠血清胆固醇的浓度。此外，通过增加ABCG5和ABCG8的表达，E.AceCalis ATCC19433降低了肝胆固醇的浓度和改善胆固醇。此外，口服施用E.AceCalis ATCC19433调节了肠道微生物的组成，增加了乳酸杆菌，双歧杆菌和Akkermansia的计数。我们的研究结果表明，通过改善转运蛋白ABCG5和ABCG8，E. Faecalis ATCC19433可以对高胆固醇小鼠发挥脱粒性血糖作用。 E. FAECALIS ATCC19433可能有助于胆固醇的运输，并调节肠道微生物的组成。

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