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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Radiation Gene-expression Signatures in Primary Breast Cancer Cells
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Radiation Gene-expression Signatures in Primary Breast Cancer Cells

机译：原发性乳腺癌细胞中的辐射基因表达鉴定

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摘要

Background/aim: In breast cancer (BC) care, radiation therapy (RT) is an efficient treatment to control localized tumor. Radiobiological research is needed to understand molecular differences that affect radiosensitivity of different tumor subtypes and the response variability. The aim of this study was to analyze gene expression profiling (GEP) in primary BC cells following irradiation with doses of 9 Gy and 23 Gy delivered by intraoperative electron radiation therapy (IOERT) in order to define gene signatures of response to high doses of ionizing radiation. Materials and Methods: We performed GEP by cDNA microarrays and evaluated cell survival after IOERT treatment in primary BC cell cultures. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to validate candidate genes. Results: We showed, for the first time, a 4-gene and a 6-gene signature, as new molecular biomarkers, in two primary BC cell cultures after exposure at 9 Gy and 23 Gy respectively, for which we observed a significantly high survival rate. Conclusion: Gene signatures activated by different doses of ionizing radiation may predict response to RT and contribute to defining a personalized biological-driven treatment plan.
机译：背景/目的：在乳腺癌（BC）护理中，放射治疗（RT）是控制局部肿瘤的有效处理。需要辐射生物学研究来了解影响不同肿瘤亚型的放射敏感性的分子差异以及响应变异性。本研究的目的是在用术中电子放射治疗（IOERT）递送的剂量9GY和23 GY照射后分析原发性BC细胞中的基因表达分析（GEP），以定义对高剂量电离的响应的基因特征辐射。材料和方法：通过CDNA微阵列进行GEP，并在原发性BC细胞培养物中进行IOERT治疗后评估细胞存活。进行实时定量逆转录聚合酶链反应（QRT-PCR）以验证候选基因。结果：我们首次表明了4-基因和6-基因签名，作为新的分子生物标志物，分别在9 GY和23 GY的曝光后的两个主要BC细胞培养物中，我们观察到显着高的存活率速度。结论：由不同剂量的电离辐射激活的基因签名可以预测对RT的反应，并有助于定义个性化生物驱动的治疗计划。

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