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首页> 外文期刊>Journal of biochemical and molecular toxicology >Ferutinin: A phytoestrogen from ferula and its anticancer, antioxidant, and toxicity properties
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Ferutinin: A phytoestrogen from ferula and its anticancer, antioxidant, and toxicity properties

机译：Ferutinin：来自Ferula的植物雌激素及其抗癌，抗氧化和毒性特性

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摘要

This study was performed to evaluate the antioxidant, anticancer, and toxicity properties of ferutinin, a phytoestrogen derived from Ferula species. The human Michigan Cancer Foundation-7 (MCF-7) breast cancer cell line and normal human fibroblast (HDF) were cultured and treated with different ferutinin concentrations. The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell death-defining tests (a comparative real-time polymerase chain reaction [for Bax and Bcl-2 genes], flow cytometry, and acridine orange/propidium iodide cell staining). Moreover, 15 white male balb/c mice were divided into three groups of five (one untreated control group and two groups), which received different doses of ferutinin-supplemented water (500 and 1000 μg/kg mice weight) to check the mice liver and kidney pathomorphological alterations and to determine the antioxidant enzymes' expression profile (superoxide dismutase [SOD], catalase [CAT], and glu-tathione peroxidase) in the mentioned tissues. Finally, the liver lipid peroxidation of mice was analyzed. The results of MTT and cell death-defining tests indicate the significant reduction in cell viability and induction of apoptotic death in MCF-7 cells (enhanced sub-Gl peaks, Bax overexpression, Bcl-2 downregulation, and increased apoptotic cells). The antioxidant enzymes (SOD and CAT) in the mice liver and kidney cells were found to be upregulated (p<.05) in response to the increasing doses of ferutinin. Besides, the lipid peroxidation of the liver tissue of mice was significantly reduced. According to the results, we suggest that ferutinin has the potential to be served as a selective anticancer compound for breast cancer treatment.
机译：本研究旨在评估阿魏素（一种从阿魏属植物中提取的植物雌激素）的抗氧化、抗癌和毒性特性。培养人密歇根癌症基金会-7（MCF-7）乳腺癌细胞系和正常人成纤维细胞（HDF），并用不同浓度的阿魏苷处理。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化铵（MTT）测定和细胞死亡定义试验（比较实时聚合酶链反应[Bax和Bcl-2基因]、流式细胞术和吖啶橙/碘化丙啶细胞染色）评估细胞活力。此外，15只雄性balb/c小鼠被分为三组，每组五只（一个未经治疗的对照组和两个组），其接受不同剂量的添加阿魏素的水（500和1000μg/kg小鼠体重），以检查小鼠肝脏和肾脏的病理形态学改变，并确定所述组织中抗氧化酶的表达谱（超氧化物歧化酶[SOD]、过氧化氢酶[CAT]和谷氨硫酮过氧化物酶）。最后，对小鼠肝脏脂质过氧化进行了分析。MTT和细胞死亡定义试验的结果表明，MCF-7细胞的细胞活力显著降低，并诱导凋亡性死亡（亚Gl峰增强，Bax过度表达，Bcl-2下调，凋亡细胞增多）。随着剂量的增加，小鼠肝和肾细胞中的抗氧化酶（SOD和CAT）被发现上调（p<0.05）。此外，小鼠肝组织脂质过氧化显著降低。根据研究结果，我们认为，阿魏苷有可能成为乳腺癌治疗的选择性抗癌化合物。

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