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首页> 外文期刊>Applied Microbiology and Biotechnology >A recombinant porcine circovirus type 2 expressing the VP1 epitope of the type O foot-and-mouth disease virus is infectious and induce both PCV2 and VP1 epitope antibodies
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A recombinant porcine circovirus type 2 expressing the VP1 epitope of the type O foot-and-mouth disease virus is infectious and induce both PCV2 and VP1 epitope antibodies

机译：表达O型口蹄疫病毒VP1表位的重组猪圆环病毒2型具有传染性，可诱导PCV2和VP1表位抗体

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摘要

Porcine circovirus type 2 (PCV2) is the etiological agent of postweaning multisystemic wasting syndrome, a disease that causes huge economic damage in swine industry. A recombinant PCV2 expressing the neutralizingVP1 epitope (aa 141–160) of the foot-and-mouth disease virus (FMDV) was rescued using an infectious cloning technique. The PCV2 antigen and FMDV-VP1 antigenic epitope of the cloned strain recPCV2-CL-VP1 were confirmed by an immunoperoxidase monolayer assay (IPMA) and a capture enzyme-linked immunosorbent assay (ELISA). The morphological features of the recPCV2-CL-VP1 were not discernibly different from those of its parental strain (PCV2-CL). However, the recombinant virus could be differentiated from its parental virus by PCR and capture ELISA. The recPCV2-CL-VP1 was demonstrated to replicate stably in PK-15 cells through ten passages. An infection experiment using BALB/c mice showed that both recPCV2-CL-VP1 and PCV2-CL could replicate in the mice, cause various pathological changes, and induce a high level of anti-Cap antibodies. The recombinant virus emulsified with Freund’s adjuvant was used to immunize BALB/c mice and induced antibodies against the FMDV-VP1 epitope. Hence, the recombinant PCV2 strain, which expressed the neutralizing FMDV-VP1 epitope, provides a valuable platform to develop novel genetic vaccines.
机译：猪圆环病毒2型（PCV2）是断奶后多系统消耗综合症的病原体，该疾病在养猪业中造成巨大的经济损失。使用感染性克隆技术拯救了表达口蹄疫病毒（FMDV）中和性VP1表位（aa 141-160）的重组PCV2。克隆的recPCV2-CL-VP1菌株的PCV2抗原和FMDV-VP1抗原表位通过免疫过氧化物酶单层测定（IPMA）和捕获酶联免疫吸附测定（ELISA）进行确认。 recPCV2-CL-VP1的形态特征与其亲本菌株（PCV2-CL）的形态特征没有明显区别。但是，重组病毒可以通过PCR和捕获ELISA与亲本病毒区分开。证实recPCV2-CL-VP1可通过十次传代在PK-15细胞中稳定复制。使用BALB / c小鼠进行的感染实验表明recPCV2-CL-VP1和PCV2-CL均可在小鼠中复制，引起各种病理变化并诱导高水平的抗Cap抗体。用弗氏佐剂乳化的重组病毒用于免疫BALB / c小鼠，并诱导出针对FMDV-VP1表位的抗体。因此，表达中和FMDV-VP1表位的重组PCV2菌株提供了开发新型遗传疫苗的宝贵平台。

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