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首页> 外文期刊>Applied Microbiology and Biotechnology >Nitrite metabolism in Debaryomyces hansenii TOB-Y7, a yeast strain involved in tobacco fermentation
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Nitrite metabolism in Debaryomyces hansenii TOB-Y7, a yeast strain involved in tobacco fermentation

机译：涉及烟草发酵的酵母菌株汉逊德巴利酵母TOB-Y7中的亚硝酸盐代谢

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摘要

The Italian cigar manufacturing process includes a fermentation step that leads to accumulation of nitrite and tobacco-specific nitrosamines (TSNA), undesirable byproducts due to their negative impact on health. In this study, growth and biochemical properties of Debaryomyces hansenii TOB-Y7, a yeast strain that predominates during the early phase of fermentation, have been investigated. With respect to other D. hansenii collection strains (Y7426, J26, and CBS 1796), TOB-Y7 was characterized by the ability to tolerate very high nitrite levels and to utilize nitrite, but not nitrate, as a sole nitrogen source in a chemically defined medium, a property that was enhanced in microaerophilic environment. The ability to assimilate nitrite was associated to the presence of YNI1, the gene encoding the assimilatory NAD(P)H:nitrite reductase (NiR), absent in Y7426, J26, and CBS 1796 by Southern blot data. YNI1 from TOB-Y7 was entirely sequenced, and its expression was analyzed in different media by Northern blot and reverse transcriptase polymerase chain reaction. The evidence that, in D. hansenii TOB-Y7, YNI1 was transcriptional active also in the presence of high ammonia concentration typical of tobacco fermentation, stimulated the development of an improved process that, on a laboratory scale, was proved to be effective in minimizing nitrite and TSNA accumulation.
机译：意大利雪茄的制造过程包括一个发酵步骤，该发酵步骤会导致亚硝酸盐和烟草特有的亚硝胺（TSNA）的积累，这些亚硝胺是不良副产物，因为它们会对健康造成负面影响。在这项研究中，已经研究了汉逊德巴利酵母TOB-Y7（一种在发酵初期占主导地位的酵母菌株）的生长和生化特性。对于其他汉森D. hansenii收集菌株（Y7426，J26和CBS 1796），TOB-Y7的特点是能够耐受非常高的亚硝酸盐水平，并且能够利用亚硝酸盐而不是硝酸盐作为化学中唯一的氮源定义的介质，在微需氧环境中增强的特性。根据DNA印迹数据，Y7426，J26和CBS 1796中不存在YNI1（编码NAD（P）H：亚硝酸盐还原酶（NiR）的同化基因）的存在，同化亚硝酸盐的能力。对来自TOB-Y7的YNI1进行了完全测序，并通过Northern印迹和逆转录聚合酶链反应在不同培养基中分析了其表达。在汉森酵母TOB-Y7中，YNI1在烟草发酵中常见的高氨浓度下也具有转录活性的证据，刺激了改进方法的发展，在实验室规模上，该方法被证明可有效地将其最小化亚硝酸盐和TSNA积累。

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