...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >The novel quinolone CHM-1 induces DNA damage and inhibits DNA repair gene expressions in a human osterogenic sarcoma cell line.
【24h】

The novel quinolone CHM-1 induces DNA damage and inhibits DNA repair gene expressions in a human osterogenic sarcoma cell line.

机译：新型喹诺酮CHM-1可以诱导人甾体肉瘤细胞系中的DNA损伤并抑制DNA修复基因的表达。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

20-Fluoro-6,7-methylenedioxy-2-phenyl-4-quino-lone (CHM-1) has been reported to induce cell cycle arrest and apoptosis in many types of cancer cells. However, there is no available information to show CHM-1 affecting DNA damage and expression of associated repair genes. Herein, we investigated whether or not CHM-1 induced DNA damage and affected DNA repair gene expression in U-2 OS human osterogenic sarcoma cells. The comet assay showed that incubation of U-2 OS cells with 0, 0.75, 1.5, 3 and 6 muM of CHM-1 led to a longer DNA migration smear (comet tail). DNA gel electrophoresis showed that 3 muM of CHM-1 for 24 and 48 h treatment induced DNA fragmentation in U-2 OS cells. Real-time PCR analysis showed that treatment with 3 muM of CHM-1 for 24 h reduced the mRNA expression levels of ataxia telangiectasia mutated (ATM), ataxia-telangiectasia and Rad3-related (ATR), breast cancer 1, early onset (BRCA1), 14-3-3sigma (14-3-3sigma), DNA-dependent serine/threonine protein kinase (DNA-PK) and O(6)-methylguanine-DNA methyltransferase (MGMT) genes in a time-dependent manner. Taken together, the results indicate that CHM-1 caused DNA damage and reduced DNA repair genes in U-2 OS cells, which may be the mechanism for CHM-1-inhibited cell growth and induction of apoptosis.
机译：据报道20-氟-6,7-亚甲基二氧基-2-苯基-4-喹啉酮（CHM-1）在许多类型的癌细胞中诱导细胞周期停滞和凋亡。但是，没有可用的信息显示CHM-1影响DNA损伤和相关修复基因的表达。在本文中，我们调查了CHM-1是否在U-2 OS人成因肉瘤细胞中诱导DNA损伤并影响DNA修复基因表达。彗星试验表明，将U-2 OS细胞与0、0.75、1.5、3和6μMCHM-1一起孵育会导致更长的DNA迁移涂片（彗尾）。 DNA凝胶电泳显示3μMCHM-1分别在24 h和48 h诱导U-2 OS细胞DNA断裂。实时PCR分析显示，用3μMCHM-1处理24小时可降低共济失调毛细血管扩张突变（ATM），共济失调毛细血管扩张和Rad3相关（ATR），乳腺癌1，早发（BRCA1）的mRNA表达水平。 ），14-3-3sigma（14-3-3sigma），DNA依赖的丝氨酸/苏氨酸蛋白激酶（DNA-PK）和O（6）-甲基鸟嘌呤-DNA甲基转移酶（MGMT）基因以时间依赖的方式。两者合计，结果表明CHM-1导致U-2 OS细胞中的DNA损伤和减少的DNA修复基因，这可能是CHM-1抑制细胞生长和诱导凋亡的机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号