...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Surface expression and CEA binding of hnRNP M4 protein in HT29 colon cancer cells.
【24h】

Surface expression and CEA binding of hnRNP M4 protein in HT29 colon cancer cells.

机译：hnRNP M4蛋白在HT29结肠癌细胞中的表面表达和CEA结合。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Carcinoembryonic antigen (CEA) has been shown to participate in the progression and metastatic growth of colorectal cancer. However, its biological function remains elusive. Recently, we found that CEA protects colon cancer cells from undergoing apoptosis, suggesting a complex role that includes signal transduction activity. Additionally, it was reported that CEA binds to Kupffer cells and macrophages to a membrane-anchored homolog of heterogeneous nuclear protein M4 (hnRNP M4), which subsequently was named CEA-receptor (CEAR). Cytoplasmatic and membranous expression of CEAR in CEA-positive colon cancer tissues prompted us to analyze the CEA-CEAR interaction in HT29 colon cancer cells. Both, CEA and CEAR were found on the cell surface of HT29 cells, as demonstrated by confocal microscopy. Imaging analysis suggested co-localization and, thus, interaction of both molecules. To confirm this observation, immunoprecipitation experiments and Western blot analysis were performed and indicated binding of CEA and CEAR. Immunoprecipitation of CEA resulted in a pull down of CEAR. The pull down of CEAR correlated with the amount of CEA as demonstrated by ribozyme targeting of CEA. Finally, external treatment of HT29 cells with soluble CEA induced tyrosine phosphorylation of CEAR, suggesting a CEA-dependent role of CEAR in signal transduction. Future experiments will elucidate whether the CEA-CEAR interaction is involved in CEA's antiapoptotic role and mediates the prometastatic properties of CEA in colon cancer cells.
机译：癌胚抗原（CEA）已显示参与大肠癌的进展和转移性生长。但是，其生物学功能仍然难以捉摸。最近，我们发现CEA保护结肠癌细胞免于凋亡，提示其复杂的作用包括信号转导活性。此外，据报道，CEA与库普弗细胞结合，巨噬细胞与异种核蛋白M4（hnRNP M4）的膜锚定同源物结合，后者后来被称为CEA受体（CEAR）。 CEA阳性结肠癌组织中CEAR的胞质和膜表达促使我们分析HT29结肠癌细胞中CEA-CEAR的相互作用。共聚焦显微镜证实，CEA和CEAR均在HT29细胞的细胞表面上发现。成像分析表明共定位，因此，两个分子的相互作用。为了证实该观察结果，进行了免疫沉淀实验和蛋白质印迹分析，并表明了CEA和CEAR的结合。 CEA的免疫沉淀导致CEAR下降。 CEAR的下降与CEA的量相关，正如核酶靶向CEA所证明的那样。最后，用可溶性CEA对HT29细胞进行外部处理可诱导CEAR的酪氨酸磷酸化，表明CEAR在信号转导中具有CEA依赖性作用。未来的实验将阐明CEA-CEAR相互作用是否与CEA的抗凋亡作用有关，并介导CEA在结肠癌细胞中的转移能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号