Use of inverse PCR for analysis of class 1 integrons carrying an unusual 3' conserved segment structure.

Lee MF; Peng CF; Hsu HJ; Toh HS

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Use of inverse PCR for analysis of class 1 integrons carrying an unusual 3' conserved segment structure.

机译：反向PCR用于分析携带不寻常的3'保守区段结构的1类整合素。

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By using inverse PCR and DNA sequencing, 13 sul3-associated mutational integrons, 2 defective class 1 integrons, and 1 qnrB2-associated complex sul1-type class 1 integrons were identified in Salmonella enterica serovar Choleraesuis, Pseudomonas aeruginosa, and Enterobacter cloacae, respectively. In addition, conjugation and Southern hybridization demonstrated that unusual class 1 integrons were located on plasmids or integrated into chromosomal DNA. Thus, an inverse PCR assay can be a valuable tool for the analysis of unusual structures of the 3' conserved region of class 1 integrons.

机译：通过反向PCR和DNA测序，分别在肠炎沙门氏菌，铜绿假单胞菌和阴沟肠杆菌中鉴定了13个sul3相关的突变整合素，2个有缺陷的1类整合素和1个与qnrB2相关的复杂sul1型1类整合素。此外，结合和Southern杂交表明不寻常的1类整合素位于质粒上或整合到染色体DNA中。因此，反向PCR测定法可以是用于分析1类整联蛋白的3'保守区的异常结构的有价值的工具。

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《Antimicrobial agents and chemotherapy.》 |2011年第2期|共3页
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Lee MF; Peng CF; Hsu HJ; Toh HS;
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正文语种 eng
中图分类治疗学;
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1. Use of inverse PCR for analysis of class 1 integrons carrying an unusual 3' conserved segment structure. [J] . Lee MF, Peng CF, Hsu HJ, Antimicrobial agents and chemotherapy. . 2011,第2期

机译：反向PCR用于分析携带不寻常的3'保守区段结构的1类整合素。
2. Salmonella entehca serovar Typhimurium Virulence-Resistance Plasmids Derived from the pSLT Carrying Nonconventional Class 1 Integrons with dfrA12 Gene in Their Variable Region and sul3 in the 3' Conserved Segment [J] . Janine Beutlich, M. Rosario Rodicio, M. Carmen Mendoza, Microbial drug resistance: MDR : Mechanisms, epidemiology, and disease . 2014,第6期

机译：沙门氏菌血清型鼠伤寒沙门氏菌抗毒力质粒衍生自携带非常规1类整合素的pSLT，其可变区带有dfrA12基因，而3'保守区段中含有sul3。
3. Salmonella entehca serovar Typhimurium Virulence-Resistance Plasmids Derived from the pSLT Carrying Nonconventional Class 1 Integrons with dfrA12 Gene in Their Variable Region and sul3 in the 3' Conserved Segment [J] . Janine Beutlich, M. Rosario Rodicio, M. Carmen Mendoza, Microbial drug resistance: MDR : Mechanisms, epidemiology, and disease . 2013,第6期

机译：Salmonella Entehca Serovar Typhimurium毒力抗性质粒衍生自PSLT在其可变区和SUL3中与DFRA12基因的PSLT携带非共同级别1整合单位在3'保守区段中
4. Distribution and Sequencing of Class 1 Integrons Carrying qac Gene Cassettes in Environment Sample [C] . Duan Xuejun, Gong Weijin 2010 4th International Conference on Bioinformatics and Biomedical Engineering . 2010

机译：携带qac基因盒的1类整合子在环境样品中的分布与测序。
5. Quantification and Correlation Analysis of Antibiotic Resistance Gene, ermF, and Class 1 Integron, intI1, in Commercially Available Fertilizers [D] . Callejas, Ileana. 2019

机译：市售肥料中抗生素抗性基因，ERMF和1级整合onton，INTI1的定量和相关分析
6. Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3′ Conserved Segment Structure [O] . Mei-Feng Lee, Chien-Fang Peng, Hui-Jine Hsu, 2011

机译：反向PCR用于分析携带异常3保守区段结构的1类整合素
7. Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3′ Conserved Segment Structure▿ [O] . Lee, Mei-Feng, Peng, Chien-Fang, Hsu, Hui-Jine, 2010

机译：使用反向PCR分析携带异常3'保守区段结构的1类整合素▿

Use of inverse PCR for analysis of class 1 integrons carrying an unusual 3' conserved segment structure.

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