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首页> 外文期刊>Antimicrobial agents and chemotherapy. >The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168.
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The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168.

机译：较大的机械敏感通道MscL确定细菌对细菌素sublancin 168的敏感性。

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摘要

Bacillus subtilis strain 168 produces the extremely stable and broad-spectrum lantibiotic sublancin 168. Known sublancin 168-susceptible organisms include important pathogens, such as Staphylococcus aureus. Nevertheless, since its discovery, the mode of action of sublancin 168 has remained elusive. The present studies were, therefore, aimed at the identification of cellular determinants for bacterial susceptibility toward sublancin 168. Growth inhibition and competition assays on plates and in liquid cultures revealed that sublancin 168-mediated growth inhibition of susceptible B. subtilis and S. aureus cells is affected by the NaCl concentration in the growth medium. Added NaCl did not influence the production, activity, or stability of sublancin 168 but, instead, lowered the susceptibility of sensitive cells toward this lantibiotic. Importantly, the susceptibility of B. subtilis and S. aureus cells toward sublancin 168 was shown to depend on the presence of the large mechanosensitive channel of conductance MscL. In contrast, MscL was not involved in susceptibility toward the bacteriocin nisin or Pep5. Taken together, our unprecedented results demonstrate that MscL is a critical and specific determinant in bacterial sublancin 168 susceptibility that may serve either as a direct target for this lantibiotic or as a gate of entry to the cytoplasm.
机译：枯草芽孢杆菌菌株168产生极其稳定和广谱的羊毛硫抗生素sublancin168。已知对sublancin 168敏感的生物包括重要的病原体，例如金黄色葡萄球菌。然而，自发现以来，Sublancin 168的作用方式仍然难以捉摸。因此，本研究旨在鉴定细菌对sublancin 168敏感性的细胞决定因素。在平板和液体培养物中的生长抑制和竞争试验表明，sublancin 168介导了对敏感枯草芽孢杆菌和金黄色葡萄球菌细胞的生长抑制。受生长培养基中NaCl浓度的影响。添加的NaCl不会影响sublancin 168的产生，活性或稳定性，但是会降低敏感细胞对该羊毛硫抗生素的敏感性。重要的是，已证明枯草芽孢杆菌和金黄色葡萄球菌细胞对sublancin 168的敏感性取决于电导MscL的大机械敏感性通道的存在。相反，MscL不参与对细菌素乳链菌肽或Pep5的敏感性。两者合计，我们空前的结果表明MscL是细菌sublancin 168敏感性的关键和特定决定因素，可作为该羊毛硫抗生素的直接靶点或进入细胞质的大门。

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