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首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Breeding of D(–)-Lactic Acid High Producing Strain by Low-energy Ion Implantation and Preliminary Analysis of Related Metabolism
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Breeding of D(–)-Lactic Acid High Producing Strain by Low-energy Ion Implantation and Preliminary Analysis of Related Metabolism

机译：低能离子注入法选育D（-）-乳酸高产菌株及其相关代谢的初步分析

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摘要

The low-energy nitrogen ion beam implantation technique was used in the breeding of mutant D(–)-lactic-acid-producing strains. The wild strain Sporolactobacillus sp. DX12 was mutated by an N+ ion beam with energy of 10keV and doses ranging from 0.4 × 1015 to 6.60 × 1015 ions/cm2. Combined with an efficient screening method, an efficient mutant Y2-8 was selected after two times N+ ion beam implantation. By using the mutant Y2-8, 121.6g/l of D-lactic acid was produced with the molar yields of 162.1% to the glucose. The yield of D-lactic acid by strain Y2-8 was 198.8% higher than the wild strain.Determination of anaerobic metabolism by Biolog MT2 was used to analyze the activities of the concerned enzymes in the lactic acid metabolic pathway. The results showed that the activities of the key enzymes responded on the substrates such as 6-phosphofructokinase,pyruvate kinase, and D-lactate dehydrogenase were considerably higher in the mutants than the wild strain. These might be affected by ion beam implantation.
机译：低能氮离子束注入技术被用于生产D（–）-乳酸突变菌株的育种。野生菌株Sporolactobacillus sp.。 DX12被N +离子束诱变，能量为10keV，剂量范围为0.4×1015到6.60×1015离子/ cm2。结合有效的筛选方法，在两次N +离子束注入后，选择了有效的突变体Y2-8。通过使用突变体Y2-8，产生了121.6g / l的D-乳酸，相对于葡萄糖的摩尔产率为162.1％。 Y2-8菌株的D-乳酸产率比野生菌株高198.8％。采用Biolog MT2测定厌氧代谢，分析了相关酶在乳酸代谢途径中的活性。结果表明，突变体中关键酶的活性对底物有反应，如6-磷酸果糖激酶，丙酮酸激酶和D-乳酸脱氢酶比野生株高得多。这些可能会受到离子束注入的影响。

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