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A study on the production of citrinin by Monascus spp

机译:红曲菌生产柠檬素的研究

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The mycotoxin citrinin was detected by TLC and by HPLC in Monascus cultured on "red rice", collected from various manufacturers. Most "red rice" samples (n=32) contained citrinin in a range from 0.2 mg/kg to 140 mg/kg, while citrinin was not detected in 3 "red rice" samples. Monascus strains were isolated from these samples of "red rice", and some strains (M. anka and M. ruber) were identified as potential citrinin producers when cultivated in YES medium. Cultivation of one strain in solid and submerged cultures (synthetic medium) was carried out to aim at citrinin-free production of Monascus pigment. When monosodium glutamate or histidine were used as the sole nitrogen source to cultivate M. ruber strain JH-2 (a potential citrinin producer in liquid culture), no citrinin was detected in the fermented broth, while fermentation on rice or wheat resulted in high toxin concentrations. Aspects of citrinin determination methods (TLC, HPLC) for high colour value "red rice" samples are discussed.
机译:通过TLC和HPLC在从不同厂家收集的“红米”上培养的红曲霉中检测到真菌毒素西林蛋白。大多数“红米”样品(n = 32)中的桔皮素含量在0.2 mg / kg至140 mg / kg的范围内,而在3个“红米”样品中未检出橘皮苷。从这些“红米”样品中分离出红曲菌菌株,当在YES培养基中培养时,某些菌株(M. anka和M. ruber)被鉴定为潜在的柠檬素生产者。进行了在固体和浸没培养物(合成培养基)中的一种菌株的培养,目的是生产无桔霉素的红曲霉色素。当使用谷氨酸钠或组氨酸作为唯一的氮源来培养M. ruber菌株JH-2(液体培养中潜在的柠檬素生产者)时,在发酵液中未检测到柠檬素,而在大米或小麦上发酵会产生高毒素浓度。讨论了用于高色值“红米”样品的柠檬素测定方法(TLC,HPLC)的各个方面。

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