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首页> 外文期刊>Arthritis and Rheumatism >Impaired suppression of synovial fluid CD4+CD25- T cells from patients with juvenile idiopathic arthritis by CD4+CD25+ Treg cells.
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Impaired suppression of synovial fluid CD4+CD25- T cells from patients with juvenile idiopathic arthritis by CD4+CD25+ Treg cells.

机译：CD4 + CD25 + Treg细胞对幼年特发性关节炎患者滑液CD4 + CD25-T细胞的抑制作用减弱。

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OBJECTIVE: Natural CD4+CD25+FoxP3+ Treg cells play a crucial role in maintaining immune homeostasis and controlling autoimmunity. In patients with juvenile idiopathic arthritis (JIA), inflammation occurs despite the increased total numbers of Treg cells in the synovial fluid (SF) compared to the peripheral blood (PB). This study was undertaken to investigate the phenotype of CD4+ T cells in PB and SF from JIA patients, the function of synovial Treg cells, and the sensitivity of PB and SF CD4+CD25- effector T cells to the immunoregulatory properties of Treg cells, and to study the suppression of cytokine secretion from SF effector T cells by Treg cells. METHODS: The phenotypes of effector T cells and Treg cells of PB and SF from JIA patients and healthy donors were determined by flow cytometry. The functionality of isolated Treg cells and effector T cells was quantified in (3) H-thymidine proliferation assays. Cytokine levels were analyzed using Bio-Plex Pro assay. RESULTS: Compared to PB, SF showed significantly elevated numbers of activated and differentiated CD4+CD45RO+ T cells. Sensitivity of SF effector T cells to the suppressive effects of Treg cells from both PB and SF was impaired, correlating inversely with the expression of CD69 and HLA-DR. However, SF effector T cell cytokine secretion was partly suppressed by SF Treg cells. CONCLUSION: Our findings indicate that regulation is impaired in the SF of patients with JIA, as shown by the resistance of effector T cells to immunoregulation by functional Treg cells. This resistance of the SF effector T cells might be due to their activated phenotype.
机译：目的：天然CD4 + CD25 + FoxP3 + Treg细胞在维持免疫稳态和控制自身免疫中起关键作用。在幼年特发性关节炎（JIA）患者中，尽管滑液（SF）中的Treg细胞总数比外周血（PB）增加，但仍会发生炎症。这项研究旨在调查JIA患者的PB和SF中CD4 + T细胞的表型，滑膜Treg细胞的功能以及PB和SF CD4 + CD25-效应T细胞对Treg细胞免疫调节特性的敏感性，以及研究Treg细胞对SF效应T细胞抑制细胞因子分泌的作用。方法：应用流式细胞仪检测JIA患者和健康供者的PB和SF效应T细胞和Treg细胞的表型。在（3）H-胸苷增殖测定中定量分离的Treg细胞和效应T细胞的功能。使用Bio-Plex Pro测定法分析细胞因子水平。结果：与PB相比，SF显示出活化和分化的CD4 + CD45RO + T细胞数量明显增加。 SF效应T细胞对来自PB和SF的Treg细胞抑制作用的敏感性受损，与CD69和HLA-DR的表达呈负相关。但是，SF Treg细胞可部分抑制SF效应T细胞的细胞因子分泌。结论：我们的发现表明，JIA患者的SF调节受到损害，如效应T细胞对功能性Treg细胞的免疫调节的抗性所示。 SF效应T细胞的这种抗性可能是由于其活化的表型。

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