首页> 外文期刊>Biochimica et biophysica acta: international journal of biochemistry and biophysics >Functional analysis of the 5'-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT.
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Functional analysis of the 5'-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT.

机译:人丙氨酸:乙醛酸氨基转移酶基因AGXT 5'侧翼区的功能分析。

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摘要

Primer extension of human liver poly(A)(+) RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5'-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.
机译:人肝poly(A)(+)RNA的引物延伸显示,人丙氨酸:乙醛酸氨基转移酶基因(AGXT)的主要转录起始位点位于翻译起始位点上游45 bp附近。使用AGXT基因的1203 bp 5'侧翼区域和荧光素酶报道基因进行的缺失分析表明,启动子序列最有可能位于翻译起始位点上游2-325 bp,可能具有增强子元件上游440-700 bp。还暗示了-2至-64区域对于AGXT基因的表达很重要。区域-2至-325具有两个TATA框和一些启动器元素。

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