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首页> 外文期刊>Asian Fisheries Science >PHA and Con A as mitogenic factors for the cultivation of lymphocytes of catfishes
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PHA and Con A as mitogenic factors for the cultivation of lymphocytes of catfishes

机译：PHA和Con A作为有丝分裂因子用于cat鱼淋巴细胞的培养

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摘要

The lymphocytes of Heteropneustes fossilis and Clarias batrachus were isolated by monoculture (centrifugation of diluted blood or Plasmagel method) and Mixed lymphocyte culture (cultured directly from the spleen) and comparative efficacy of both methods were discussed. The lymphocytes were cultured in two growth media, medium A (mitogen Con A; antibiotic penicillin) and medium B (mitogen PHA; antibiotic streptomycin) in the medium RPMI 1640 with 10% FCS and HEPES buffer. The samples were assessed for lymphocyte growth every 24 h for 96 h. The results indicated that PHA is a superior mitogen and MLC is a better technique for causing blastogenesis and proliferation of lymphocytes.
机译：通过单培养（稀释血液离心或Plasmagel方法）和混合淋巴细胞培养（直接从脾脏培养）分离出Heteropneustes化石和Clarias batrachus的淋巴细胞，并讨论了这两种方法的比较效果。在含有10％FCS和HEPES缓冲液的RPMI 1640培养基中的两种生长培养基中，分别在培养基A（促分裂原Con A;抗生素青霉素）和培养基B（促分裂原PHA;抗生素链霉素）中培养淋巴细胞。每24小时评估样品的淋巴细胞生长，持续96小时。结果表明，PHA是一种优良的促分裂原，而MLC是一种更好的引起胚细胞增殖和淋巴细胞增殖的技术。

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