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斑马鱼B淋巴细胞刺激因子cDNA及其克隆方法和重组应用

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摘要

斑马鱼B淋巴细胞刺激因子（zBAFF)cDNA及其克隆方法和重组应用，涉及生物基因工程领域。斑马鱼B淋巴细胞刺激因子基因的克隆方法如下：TRIzol法提取斑马鱼脾脏总RNA并进行逆转录；通过Clustalw软件分析虹鳟鱼,大西洋鲑鱼,斑点绿河豚BAFFcDNA以及虹鳟鱼，大西洋鲑鱼，斑点叉尾鮰BAFFcDNA保守区设计兼并引物进行PCR扩增，获得807bp全长cDNA。所述斑马鱼B淋巴细胞刺激因子和增殖诱导配体cDNA可通过现有基因工程方法，生产重组斑马鱼的BAFF。

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公开/公告号CN102115748A

专利类型发明专利
公开/公告日2011-07-06

原文格式PDF
申请/专利权人南京师范大学;
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申请/专利号CN201010598396.6
发明设计人张双全;闵翠;梁臻宁;
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申请日2010-12-21
分类号C12N15/12;C12N15/10;A61K48/00;A61P37/04;C07K14/52;
代理机构南京知识律师事务所;
代理人卢亚丽
地址 210046 江苏省南京市亚东新城区文苑路1号
入库时间 2023-12-18 02:47:37

法律信息

法律状态公告日

法律状态信息

法律状态
2013-02-13

发明专利申请公布后的视为撤回 IPC(主分类):C12N15/12 申请公布日:20110706 申请日:20101221

发明专利申请公布后的视为撤回
2011-08-24

实质审查的生效 IPC(主分类):C12N15/12 申请日:20101221

实质审查的生效
2011-07-06

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1. 斑马鱼B淋巴细胞刺激因子cDNA及其克隆方法和重组应用 [P] . 中国专利： CN102115748A . 2011-07-06
2. 狗B淋巴细胞刺激因子cDNA及其克隆方法和重组应用 [P] . 中国专利： CN102121012A . 2011-07-13
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机译：从空肠吸虫唾液腺cdna文库获得kunitz蛋白酶抑制剂的方法：克隆寡核苷酸序列和重组蛋白氨基酸序列，重组蛋白;测定对活化因子x的抑制活性的方法，测定血浆中抗凝活性的方法，测定人和鼠肿瘤细胞系中凋亡活性的方法，测定黑素瘤肿瘤中抗转移活性的方法，体外方法和体内抗癌活性的测定，重组用于不同的稳态（凝血和免疫反应），抗增殖，抗凋亡和抗血管生成机制