• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Australian Journal of Dairy Technology >Transmission electron microscopy imaging of the microstructure of milk in cheddar cheese production under different processing conditions.
【24h】

Transmission electron microscopy imaging of the microstructure of milk in cheddar cheese production under different processing conditions.

机译：在不同加工条件下切达干酪生产中牛奶的微结构的透射电子显微镜成像。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Milk processing prior to cheesemaking serves a number of functions, but may also irreversibly change the structure of the milk components. The effect of processing parameters on the microstructure of several milk preparations was assessed using transmission electron microscopy (TEM). Our TEM results confirmed the presence of the native milkfat globule membrane on fat globules within raw milk, within raw milk ultrafiltration retentate and within pasteurised and standardised milk for cheddar cheesemaking. Sodium dodecyl sulphate - polyacrylamide gel electrophoresis confirmed the identity of the native MFGM proteins isolated from the surface of fat globules. In contrast, homogenisation produced the greatest changes in the microstructure of the fat globules, where the native MFGM could not be detected using TEM. The fat globules within homogenised milk had mostly casein present on the fat globule surface. These changes in the microstructure of the milk components may alter the microstructure of downstream products. Our results highlight the potential use of TEM to complement previously reported techniques such as confocal laser scanning microscopy (CLSM) to provide valuable information about membrane integrity and changes associated with milk preparation for cheddar cheese production.
机译：奶酪制作之前的牛奶加工具有许多功能，但也可能不可逆地改变牛奶成分的结构。使用透射电子显微镜（TEM）评估了加工参数对几种牛奶制品微观结构的影响。我们的TEM结果证实了天然乳脂球状膜存在于生乳中，生乳超滤渗余物中以及用于切达干酪制作的巴氏消毒和标准化乳中的脂肪小球上。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳证实了从脂肪小球表面分离出的天然MFGM蛋白的身份。相反，均质化在脂肪小球的微观结构中产生了最大的变化，而使用TEM无法检测到天然MFGM。均质牛奶中的脂肪球在脂肪球表面上大部分存在酪蛋白。牛奶成分的微观结构的这些变化可能会改变下游产品的微观结构。我们的研究结果突显了TEM潜在地补充了先前报道的技术（例如共聚焦激光扫描显微镜（CLSM））以提供有关膜完整性和与切达干酪生产的牛奶制备相关的变化的有价值的信息。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号