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Genotyping and chlorine-resistance of Methylobacterium aquaticum isolated from water samples in Japan.

机译:从日本水样中分离出的水产甲基甲烷杆菌的基因分型和耐氯性。

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For microbial ecological analysis, 14 strains of Methylobacterium aquaticum isolated from water samples were subjected to clustering analysis on the basis of ribotyping and RAPD-PCR tests. The ribopatterns after digestion with EcoRI obtained from 14 strains of M. aquaticum were used to divide the strains into two groups (Groups I and II) with a similarity of 55%. From the analysis of RAPD patterns using primer 208, the 14 strains were divided into 3 groups (A-C) based on a homology of 45% or greater, and from that using primer 272, there were 4 groups (A-D) based on a homology of 50% or greater. The chlorine resistance (99.9% CT values) of these isolates was also experimentally confirmed, and we attempted to define the connection between chlorine resistance and the geno-cluster. The average CT value of group I was 0.89 mg.min/l and the average of group II was 0.69 mg.min/l. No remarkable differences in the CT values for the groups were found.
机译:为了进行微生物生态学分析,根据核糖分型法和RAPD-PCR试验,对从水样中分离出的14株水产甲基杆菌进行了聚类分析。用14种M菌株获得的Eco RI消化后的核糖模式。使用水生植物将菌株分为相似性为55%的两组(I和II组)。根据使用引物208进行的RAPD分析,将14个菌株根据45%以上的同源性分为3个组(AC),根据使用引物272的同源性,将4个组(AD)分为4个组。 50%或更高。这些分离物的耐氯性(99.9%CT值)也已通过实验证实,我们试图确定耐氯性与基因簇之间的联系。 I组的平均CT值为0.89 mg.min / l,II组的平均CT值为0.69 mg.min / l。没有发现各组的CT值有显着差异。

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