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首页> 外文期刊>Biocontrol Science >Pulsed-Field Gel Electrophoresis (PFGE) Pattern Analysis and Chlorine-Resistance of Legionella pneumophila Isolated from Hot Spring Water Samples
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Pulsed-Field Gel Electrophoresis (PFGE) Pattern Analysis and Chlorine-Resistance of Legionella pneumophila Isolated from Hot Spring Water Samples

机译：温泉水样品中嗜肺军团菌的脉冲场凝胶电泳（PFGE）模式分析和耐氯性

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摘要

For a microbial ecological analysis, 20 strains of Legionella pneumophila isolated from both unchlorinated Noyu (unattended natural hot spring) samples and chlorinated hot spring bath water samples collected throughout Japan were subjected to a clustering analysis on the basis of a Pulsed-Field Gel Electrophoresis (PFGE) pattern analysis. The PFGE patterns obtained from 19 strains of L. pneumophila after digestion with Sfil were used to divide the strains into two groups (Groups A and B), although the similarity level was very low among the groups. Group A consisted of 8 strains, and all of these strains were isolated from hot spring bath water samples. Group B consisted of 11 strains, and all but two of these strains were isolated from Noyu samples. The chlorine resistance (99.9% CT values) of these isolates was experimentally confirmed, and we attempted to define the relationship between chlorine resistance and the geno-cluster. The average CT value of group A (8 strains from hot spring bath water) was 0.49 mg center dot min/1 and the average of group B (9 strains from Noyu samples) was 0.29 mg center dot min/1. No remarkable differences in the CT values for the groups were found. A chlorine-sensitive Noyu strain (0.14 mg center dot min/1) and a chlorine-resistant strain (0.62 mg center dot min/1) from hot spring bath water were then compared to identify any differences in their lipid composition. There was no notable difference in the ratio of saturated to unsaturated fatty acids between the chlorinesensitive and chlorine-resistant bacteria. However, the chlorine-sensitive and chlorine-resistant bacteria demonstrated differences in the relative percentages of cell wall and cell membrane fatty acids.
机译：为了进行微生物生态学分析，从全日本各地收集的未氯化Noyu（无人值守天然温泉）样本和氯化温泉浴水样本中分离出的20株嗜肺军团菌，在脉冲场凝胶电泳（ PFGE）模式分析。从Sfil消化后从19株嗜肺乳杆菌的菌株获得的PFGE模式被用于将菌株分为两组（A组和B组），尽管各组之间的相似度非常低。 A组由8个菌株组成，所有这些菌株均从温泉浴水样品中分离出来。 B组由11个菌株组成，这些菌株中只有两个是从野汤样品中分离出来的。实验证明了这些菌株的耐氯性（CT值为99.9％），我们试图确定耐氯性与基因簇之间的关系。 A组（来自温泉浴水的8个菌株）的平均CT值为0.49mg中心点min / 1，B组（来自Noyu样品的9个菌株）的平均值为0.29mg中心点min / 1。没有发现各组的CT值有显着差异。然后将来自温泉浴水中的对氯敏感的Noyu菌株（0.14 mg中心点min / 1）和对氯敏感的菌株（0.62 mg中心点min / 1）进行比较，以鉴定其脂质成分的任何差异。在对氯敏感和对氯敏感的细菌之间，饱和脂肪酸与不饱和脂肪酸的比例没有显着差异。但是，对氯敏感和对氯敏感的细菌在细胞壁和细胞膜脂肪酸的相对百分比方面表现出差异。

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