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首页> 外文期刊>Biocontrol Science >Growth Enhancement of the Halotolerant Brevibacterium sp JCM 6894 by Methionine Externally Added to a Chemically Defined Medium
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Growth Enhancement of the Halotolerant Brevibacterium sp JCM 6894 by Methionine Externally Added to a Chemically Defined Medium

机译：蛋氨酸外部添加到化学定义的培养基中的耐盐短杆菌JCM 6894的生长增强。

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摘要

We examined amino acid requirements for the growth of the halotolerant Brevibacterium sp. JCM 6894 in the absence and presence of 1.2 M NaCl in a chemically defined medium. The experiment was also carried out in the presence of 1.2 M KCl. As a result, growth was highly enhanced by methionine in the absence and presence of KCl as well as NaCl up to 1.2 M. However, growth in the presence of 150 mM methionine was repressed by leucine (up to 100 mM) and valine (up to 100 mM). Concentration-dependent growth inhibition was observed in the presence of isoleucine (up to 150 mM) and threonine (up to 300 mM). When the cells were incubated in the absence of externally added K+, growth was strongly repressed, even in the presence of 150 mM methionine. The growth, however, recovered drastically by the addition of 1 mM KCl, regardless of the presence and absence of 1.2 M NaCl. These results indicate that methionine, which seems to be symported into cytoplasm with K+, plays an important role in the growth of the strain under salt stress.
机译：我们检查了卤代短杆菌属细菌生长的氨基酸需求。在化学成分确定的培养基中，在不存在和存在1.2 M NaCl的情况下，存在JCM 6894。该实验也在1.2 M KCl的存在下进行。结果，在不存在和存在KCl以及高达1.2 M的NaCl的情况下，蛋氨酸可高度促进生长。但是，亮氨酸（高达100 mM）和缬氨酸（高达150 mM）会抑制存在150 mM蛋氨酸的生长。至100 mM）。在异亮氨酸（最高150 mM）和苏氨酸（最高300 mM）存在下观察到浓度依赖性生长抑制。当在没有外部添加的K +的情况下孵育细胞时，即使在存在150 mM蛋氨酸的情况下也强烈抑制了生长。然而，无论是否存在1.2 M NaCl，通过添加1 mM KCl都能使生长迅速恢复。这些结果表明，蛋氨酸似乎与K +一起进入细胞质，在盐胁迫下对菌株的生长起重要作用。

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