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Evaluation of a Rapid Oral Bacteria Quantification System Using Dielectrophoresis and the Impedance Measurement

机译：利用介电泳和阻抗测量评估口腔细菌快速定量系统

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To clarify the availability of the dielectrophoretic impedance measurement (DEPIM) system as the evaluator for oral care, we evaluated the usefulness of DEPIM system by comparison with the standard plate counting (SPC) method. First, the relationship between the DEPIM results and bacterial concentration measured by SPC was clarified. Next, the measurement of the microorganism number in a mixed suspension was evaluated with DEPIM and SPC. The bacterial counts with DEPIM strongly correlated with those with SPC (r(2)=0.633-0.997) and this correlation was also shown in the measurement of a mixed bacterial suspension (ranging from 10(5) to 10(8) cfu/ml) of two bacterial species. Moreover, the experiments using dissociating enzymes to eliminate the influence of the size of the bacterial aggregates demonstrated that the microbial measurement results with DEPIM are unaffected by bacterial aggregates. This study demonstrated that bacterial counts with DEPIM strongly correlated with those with SPC and were unaffected by bacterial aggregates.
机译：为了阐明介电泳阻抗测量（DEPIM）系统作为口腔护理评估器的可用性，我们通过与标准板计数（SPC）方法进行比较来评估DEPIM系统的实用性。首先，阐明了DEPIM结果与通过SPC测量的细菌浓度之间的关系。接下来，用DEPIM和SPC评估混合悬浮液中微生物数量的测量。 DEPIM细菌计数与SPC细菌计数高度相关（r（2）= 0.633-0.997），并且在混合细菌悬浮液的测量中也显示了这种相关性（范围从10（5）到10（8）cfu / ml ）的两种细菌。此外，使用解离酶消除细菌聚集体大小的影响的实验表明，DEPIM的微生物测量结果不受细菌聚集体的影响。这项研究表明，DEPIM的细菌计数与SPC的细菌计数高度相关，并且不受细菌聚集的影响。

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