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首页> 外文期刊>Biocontrol Science >The control of the production and secretion of extracellular ice-nucleating material of Erwinia uredovora KUIN-3
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The control of the production and secretion of extracellular ice-nucleating material of Erwinia uredovora KUIN-3

机译：产酸欧文氏菌KUIN-3细胞外冰核物质的产生和分泌的控制

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摘要

The secretion of extracellular ice-nucleating material (ELM) with ice nucleation proteins (INP) from an ice-nucleating bacterium, Erwinia uredovora KUIN-3, increased in proportion to the yeast extract concentration in the ice-nucleating medium. The level of this secretion could be exhibited in terms of the ice-nucleating temperature, T_(50)(deg C), using the ELIZA method with the anti-Ina A antiserum. The secretions and the production on the cell surface of all ElM containing the class A, B and C structures were activated by the addition of yeast extract. Furthermore, based on the examination involving the deletion of various medium components, it was found that lysine (Lys) was closely associated with the secretions of ElM in the class A and B structures. The addition of Lys could affect the polyamine content in the ElM, thereby changing the surface charge of ElM from 5.2 to 4.9. Also, the addition of cadaverine (CAD) could enhance the ElM secretion. Based on these results, the surface charge of the INP aggregate was shown to be essential for the translocation of INP and the secretion of ElM. We found that this secretion, especially the secretion of a class B structure, was inhibited by N,N'dicyclohexylcarbodiimide (DCCD), which was an H~+-ATPase inhibitor regardless of normal growth. We also found that this secretion required ATP and the positive charge on the ElM surface. This is the first report on the energy needed to secrete ElM into the culture broth and the possibility for controlling the production of the INP on the cell surface by the reduction of some compounds in the surroundings.
机译：来自冰成核细菌Erwinia uredovora KUIN-3的冰成核蛋白（INP）分泌的细胞外冰成核物质（ELM）与冰成核培养基中酵母提取物的浓度成正比。使用ELIZA方法和抗Ina A抗血清，可以根据冰成核温度T_（50）（℃）来显示这种分泌水平。通过添加酵母提取物来激活所有含有A，B和C类结构的ElM的分泌和细胞表面上的产生。此外，基于涉及各种培养基成分的缺失的检查，发现赖氨酸（Lys）与A和B类结构中ElM的分泌密切相关。 Lys的添加可能会影响ElM中多胺的含量，从而将ElM的表面电荷从5.2更改为4.9。另外，添加尸胺（CAD）可以增强ElM分泌。基于这些结果，表明INP聚集体的表面电荷对于INP的移位和ElM的分泌是必不可少的。我们发现这种分泌物，特别是B类结构的分泌物，被N，N'-二环己基碳二亚胺（DCCD）抑制，该物质是H〜+ -ATPase抑制剂，无论其正常生长如何。我们还发现，这种分泌需要ATP和ElM表面的正电荷。这是有关将ElM分泌到培养液中所需能量的首次报道，以及通过减少周围环境中某些化合物来控制细胞表面INP产生的可能性。

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