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首页> 外文期刊>Indian Chemical Engineer Sections A & B >Studies on Solvent Extraction of Ethanol during Fermentation under Agitated Condition
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Studies on Solvent Extraction of Ethanol during Fermentation under Agitated Condition

机译：搅拌条件下发酵过程中乙醇的溶剂萃取研究

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摘要

In the present investigation, an attempt has been made to study the effect of agitation on simultaneous fermentation and extraction of ethyl alcohol in the presence of ethyl benzene as solvent. Three separate studies, viz. agitation of both the aqueous and non-aqueous phases at the same rotational speed of agitator (the range being 100-300 rpm), agitation of the non-aqueous phase keeping the aqueous phase static and agitation during fermentation in the absence of solvent, were carried out. In each case, Sacchoromyces cerevisiae was used as the microorganism in the production media with 10% (w/v) glucose as substrate at 30°C. It was observed that, in general, an increase in agitator speed decreased ethanol production under a solvent-free system. Under the aqueous phase static and organic phase agitated conditions, the maximum yield of ethanol in aqueous phase was obtained at a 200 rpm agitator speed after 12 h of incubation. It was further noticed that extraction of ethanol in the organic phase increased marginally when the agitator speed increased from 200 to 300 rpm after 20 h of incubation. With both phases agitated at the same agitator speed, the yield of ethanol in the organic phase increased appreciably after 200 rpm. Notably, no extraction of ethanol in organic phase was observed below 200 rpm. In the aqueous phase, the yield of ethanol consistently decreased with increase in rpm after 8 h incubation and the maximum yield was observed at agitation speed of 100 rpm at 8 h incubation.
机译：在本研究中，已经尝试研究搅拌在乙苯作为溶剂存在下同时发酵和提取乙醇的效果。三个独立的研究，即。在相同的搅拌器旋转速度（范围为100-300 rpm）下搅拌水相和非水相，保持水相静态的非水相的搅拌以及在无溶剂发酵过程中的搅拌执行。在每种情况下，酿酒酵母均被用作生产培养基中的微生物，在30°C下以10％（w / v）葡萄糖为底物。观察到，通常，在无溶剂系统下，搅拌器速度的增加会降低乙醇的产量。在水相静态和有机相搅拌的条件下，孵育12小时后，在200 rpm的搅拌器速度下，水相中乙醇的最大收率获得了。还应注意的是，孵育20小时后，当搅拌器速度从200 rpm增加到300 rpm时，有机相中乙醇的提取量略有增加。在两相以相同的搅拌器速度进行搅拌的情况下，有机相中乙醇的产率在200 rpm后显着增加。值得注意的是，在200 rpm以下未观察到有机相中乙醇的萃取。在水相中，培养8小时后，乙醇的产率随rpm的增加而持续降低，并且在培养8 h的搅拌速度为100 rpm时观察到最大产率。

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