Site-specific DNA hypomethylation permits expression of the IRBP gene.

Boatright JH; Nickerson JM; Borst DE

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Site-specific DNA hypomethylation permits expression of the IRBP gene.

机译：位点特异性DNA低甲基化可以表达IRBP基因。

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Interphotoreceptor retinoid binding protein (IRBP), a putative component of the visual cycle, is expressed selectively in the retina and pineal gland. This study examined whether site-specific DNA hypomethylation plays a role in this expression regulation. Southern blotting of HpaII and MspI digests of DNA from various bovine and murine tissues (whole brain, retina, pineal gland, superior colliculus, cortex, thymus, habenular nucleus, cornea, liver, tail, and kidney) revealed that specific CpG dinucleotides in the IRBP gene promoter are hypomethylated in DNA from retinal photoreceptor cells and pineal gland compared to DNA from other tissues. These sites are methylated in DNA from non-photoreceptor retinal cells. Exogenous methylation of these sites diminished DNA:protein binding in electrophoretic mobility shift assays. HpaII methylation of chloramphenicol acetyltransferase reporter constructs suppressed IRBP but not SV40 promoter activity in transiently transfected primary cultures of embryonic chick retinal cells. These data indicate that specific cytosines in the bovine and murine IRBP promoters are unmethylated in photoreceptive cells but methylated in other tissues. This differential DNA methylation may modulate IRBP gene expression since exogenous methylation of the murine sites suppresses reporter gene transcription, apparently by inhibiting DNA:protein binding events.

机译：感光细胞间的类维生素A结合蛋白（IRBP）是视觉周期的一个假定成分，在视网膜和松果体中选择性表达。这项研究检查了位点特异性DNA低甲基化是否在这种表达调控中起作用。各种牛和鼠组织（整个脑，视网膜，松果体，上丘，皮层，胸腺，ha状核，角膜，肝脏，尾巴和肾脏）的DNA的HpaII和MspI消化DNA的Southern印迹显示，该细胞中的特定CpG二核苷酸与来自其他组织的DNA相比，来自视网膜感光细胞和松果体的DNA中IRBP基因启动子被低甲基化。这些位点在非感光性视网膜细胞的DNA中被甲基化。这些位点的外源甲基化减少了电泳迁移率迁移分析中的DNA：蛋白结合。氯霉素乙酰转移酶报告基因构建体的HpaII甲基化抑制了瞬时转染的雏鸡视网膜细胞原代培养物中IRBP的活性，但不抑制SV40启动子的活性。这些数据表明，牛和鼠IRBP启动子中的特定胞嘧啶在感光细胞中未甲基化，但在其他组织中甲基化。这种差异性的DNA甲基化可能会调节IRBP基因的表达，因为鼠类位点的外源甲基化会抑制报告基因的转录，这显然是通过抑制DNA：蛋白质的结合事件。

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《Brain research》 |2000年第1期|共11页
作者
Boatright JH; Nickerson JM; Borst DE;
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正文语种 eng
中图分类神经病学;
关键词
DNA Methylation; Gene Expression Regulation; Photoreceptors; Pineal Body; DNA甲基化; 基因表达调控; 光感受器; 松果体; 视网膜神经节细胞; 视黄A醇结合蛋白质类;

机译：DNA Methylation;Gene Expression Regulation;Photoreceptors;Pineal Body;DNA甲基化;基因表达调控;光感受器;松果体;视网膜神经节细胞;视黄A醇结合蛋白质类;

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专利

1. Site-specific DNA hypomethylation permits expression of the IRBP gene. [J] . Boatright JH, Nickerson JM, Borst DE Brain research . 2000,第1期

机译：位点特异性DNA低甲基化可以表达IRBP基因。
2. Hypomethylation of the interphotoreceptor retinoid-binding protein (IRBP) promoter and first exon is linked to expression of the gene [J] . Adriana Albini, Douglas M. Noonan, Gerald J. Chader, Nucleic acids research . 1990,第17期

机译：受体间类视黄醇结合蛋白（IRBP）启动子和第一个外显子的次甲基化与该基因的表达有关
3. Hypomethylation of the interphotoreceptor retinoid-binding protein (IRBP) promoter and first exon is linked to expression of the gene [J] . Adriana Albini, Douglas M. Noonan, Gerald J. Chader, Nucleic acids research . 1990,第17期

机译：受体间类视黄醇结合蛋白（IRBP）启动子和第一个外显子的次甲基化与该基因的表达有关
4. Role of Humic Acids on DNA Hypomethylation Caused Fipronil Stress in Vicia faba Seedlings [C] . M. Turan, E. Arslan, G. Agar The Meeting of the International Humic Substances Society . 2015

机译：腐殖酸对DNA低甲基化的作用引起了蚕豆幼苗的FIPRONIL胁迫
5. UHRF1 overexpression causes DNA hypomethylation and liver cancer and its loss induces the interferon-gamma pathway. [D] . Mudbhary, Raksha. 2013

机译：UHRF1过表达会导致DNA甲基化不足和肝癌，其丢失会诱导干扰素-γ通路。
6. Hypomethylation of the interphotoreceptor retinoid-binding protein (IRBP) promotor and first exon is linked to expression of the gene. [O] . A Albini, J Toffenetti, Z Zhu, 1990

机译：受体间类视黄醇结合蛋白（IRBP）启动子和第一个外显子的亚甲基化与该基因的表达有关。
7. Hypomethylation of the interphotoreceptor retinoid-binding protein (IRBP) promoter and first exon is linked to expression of the gene [O] . Adriana Albini, Judith Toffenetti, Zhen Zhu, 1990

机译：反素邻吡啶结合蛋白（IRBP）启动子和第一外显子的低甲基化与基因的表达有关
8. Area- and Site-Specific Geothermal Leasing/Permitting Profiles; Updated Geothermal Leasing/Permitting Performance Assessment [R] . Beeland, G. V., Schumann, E., Wieland, M. 1982

机译：区域和地点特定的地热租赁/许可概况;更新地热租赁/许可绩效评估

Site-specific DNA hypomethylation permits expression of the IRBP gene.

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