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首页> 外文期刊>International Journal of Food Microbiology >Diversity of black Aspergilli isolated from raisins in Argentina: Polyphasic approach to species identification and development of SCAR markers for Aspergillus ibericus
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Diversity of black Aspergilli isolated from raisins in Argentina: Polyphasic approach to species identification and development of SCAR markers for Aspergillus ibericus

机译：从阿根廷的葡萄干中分离出的黑曲霉的多样性：多相法鉴定和鉴定ibericus的SCAR标记

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摘要

Aspergillus section Nigri is a heterogeneous fungal group including some ochratoxin A producer species that usually contaminate raisins. The section contains the Series Carbonaria which includes the toxigenic species Aspergillus carbonarius and nontoxigenic Aspergillus ibericus that are phenotypically undistinguishable. The aim of this study was to examine the diversity of black aspergilli isolated from raisins and to develop a specific genetic marker to distinguish A. ibericus from A. carbonarius. The species most frequently found in raisins in this study were Aspergillus tubingensis (35.4%) and A. carbonarius (32.3%), followed by Aspergillus luchuensis (10.7%), Aspergillus japonicus (7.7%), Aspergillus niger (6.2%), Aspergillus welwitschiae (4.6%) and A. ibericus (3.1%). Based on inter-simple sequence repeat (ISSR) fingerprinting profiles of major Aspergillus section Nigri members, a sequence-characterized amplified region (SCAR) marker was identified. Primers were designed based on the conserved regions of the SCAR marker and were utilized in a PCR for simultaneous identification of A. carbonarius and A. ibericus. The detection level of the SCAR-PCR was found to be 0.01 ng of purified DNA The present SCAR-PCR is rapid and less cumbersome than conventional identification techniques and could be a supplementary strategy and a reliable tool for high-throughput sample analysis. (C) 2015 Elsevier B.V. All rights reserved.
机译：黑曲霉部分Nigri是一个异质真菌群，其中包括一些通常污染葡萄干的曲霉毒素A生产者。本节包含Carbonaria系列，其中包括表型无法区分的产毒物种Carbonaria和无毒产Asibergillus ibericus。这项研究的目的是检查从葡萄干中分离出的黑曲霉的多样性，并开发出一种特定的遗传标记来区分A. ibericus和Carbonarius。在这项研究中，葡萄干中最常见的物种是油曲霉（35.4％）和碳曲霉（32.3％），其次是luchuensis（10.7％），日本曲霉（7.7％），黑曲霉（6.2％），曲霉（Aspergillus）虎杖科（4.6％）和小。菜（3.1％）。基于主要曲霉节Nigri成员的简单序列重复（ISSR）指纹图谱，鉴定了序列特征性扩增区（SCAR）标记。基于SCAR标记的保守区设计引物，并将其用于PCR中以同时鉴定碳假单胞菌和纤毛假单胞菌。发现SCAR-PCR的检测水平为0.01 ng纯化的DNA。与传统的鉴定技术相比，本SCAR-PCR快速且麻烦，可以作为高通量样品分析的补充策略和可靠工具。 （C）2015 Elsevier B.V.保留所有权利。

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