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首页> 外文期刊>International journal of immunopharmacology >Effect of combined praziquantel and recombinant glutathione S-transferase on resistance to reinfection in murine Schistosomiasis mansoni.
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Effect of combined praziquantel and recombinant glutathione S-transferase on resistance to reinfection in murine Schistosomiasis mansoni.

机译：吡喹酮和重组谷胱甘肽S-转移酶联合使用对曼氏血吸虫病鼠抗再感染的影响。

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This study was undertaken to evaluate the effect of recombinant Schistosoma mansoni-26 Glutathione S-transferase (rSm 26 GST) or soluble egg antigen (SEA) alone and in addition to praziquantel (PZQ) on the state of resistance to S. mansoni reinfection. The associated changes in the immune responses were evaluated. The experimental group of mice were injected intravenously before S. mansoni infection (80 cercariae/mouse) either with rSm26 GST (1 microgx4) or SEA (10 microgx4) in addition to PZQ (2x500 mg/kg) administered 6 weeks post-infection. Seven control groups were used, three of them were the infected (80 cercariae/mouse), the challenged (240 cercariae/mouse) and the infected challenged controls (80+240 cercariae/mouse). The rest of the four groups were the treated controls receiving: the GST-Lyzate, rSmGST, SEA and PZQ in the same doses and at the same timings. Challenge infection was conducted for all the groups 8 weeks post-infection. Animals were sacrificed 3 weeks post-challenge. After sacrifice animals were perfused and percentage resistance to reinfection was calculated. Immune responses were assessed by the measurement of hepatic granuloma diameter, intralesional T-cell phenotypes and serum immunoglobulin isotypes. The highest percentage of resistance to reinfection was observed in rGST-treated group while the lowest percentage of resistance was detected in PZQ-treated group. Whereas in mice receiving combined rGST or SEA and PZQ, percentage resistance to reinfection was significantly higher than that in PZQ treated mice. The remarkable reduction in granuloma diameter in rGST-treated group with or without PZQ was associated with decrease in the intralesional L(3)T(4)(+) and increase in Lyt(2)(+) T-cell phenotypes. However, no special relationship was observed between the percentage of resistance and the changes in granuloma diameter or intralesional T-cell phenotypes. The increase in percentage resistance to reinfection was found accompanied by increased anti SWAP IgE. Combined rGST and PZQ provided the complementary goals of improved state of resistance to reinfection 'which was compromized after cure with PZQ' and the maximal reduction in granuloma diameter.
机译：进行这项研究是为了评估重组曼氏血吸虫-谷胱甘肽-26谷胱甘肽S-转移酶（rSm 26 GST）或可溶性卵抗原（SEA）以及吡喹酮（PZQ）对沙门氏菌再感染抵抗状态的影响。评估了免疫反应的相关变化。除感染后6周施用PZQ（2x500 mg / kg）的曼氏沙门氏菌感染（80尾c /小鼠）或rSm26 GST（1 microgx4）或SEA（10 microgx4）外，对实验组小鼠进行静脉注射。使用了七个对照组，其中三个是感染的（80尾cer /小鼠），攻击的（240尾c /小鼠）和感染的对照（80 + 240尾c /小鼠）。其余四组为接受治疗的对照组，分别接受相同剂量和时间的GST-Lyzate，rSmGST，SEA和PZQ。感染后8周对所有组进行激发感染。攻击后3周处死动物。处死后，灌注动物并计算对再感染的抗性百分比。通过测量肝肉芽肿直径，病变内T细胞表型和血清免疫球蛋白同种型来评估免疫应答。在rGST治疗组中观察到最高的抵抗再感染的百分率，而在PZQ治疗组中观察到最低的抵抗百分率。而接受rGST或SEA和PZQ联合治疗的小鼠中，对再感染的抗性百分比明显高于PZQ处理的小鼠。在有或没有PZQ的rGST治疗组中，肉芽肿直径的显着减少与病灶内L（3）T（4）（+）减少和Lyt（2）（+）T细胞表型增加有关。然而，在耐药百分率与肉芽肿直径或病变内T细胞表型的变化之间未观察到特殊关系。发现对再感染的抗性百分比增加，同时抗SWAP IgE升高。结合使用rGST和PZQ提供了补充的目标，即改善了对再感染的抵抗力状态（“用PZQ治愈后就受损了”）和最大程度地降低了肉芽肿直径。

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