• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of medical microbiology: IJMM >Capsular polysaccharide gene diversity of pneumococcal serotypes 6A, 6B, 6C, and 6D
【24h】

Capsular polysaccharide gene diversity of pneumococcal serotypes 6A, 6B, 6C, and 6D

机译：肺炎球菌血清型6A，6B，6C和6D的荚膜多糖基因多样性

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study was performed to better understand the genetic diversity and evolutionary relatedness of pneumococcal serotypes 6A, 68, 6C, and 6D. Multi-locus sequence typing (MLST) was performed for 160 serogroup 6 isolates from clinical specimens collected from children between 1991 and 2010. We identified 38 sequence types (STs) comprising five clonal complexes with 12 singletons. Although most STs were confined to a single serotype, some STs were shared by two serotypes, and one ST was shared by three serotypes. Many STs of serotype 6A showed genetic relatedness with those of serotype 6C or 6D in eBURST analysis. Five capsular polysaccharide (cps) genes - wchA, wciO, wciP, wzy, and wzx - were analysed in 74 isolates from our clinical samples and in 36 isolates from GenBank. There were several profiles and clades in each serotype on the analysis of the concatenated sequences of the five cps genes. Small genetic distances between serotypes 6A and 6B and between serotypes 6C and 6D were observed while serotype 6B with an indel sequence formed a distinct clade. When comparing the individual cps genes between the serotypes, there was also a high level of similarity in the wchA and wciO gene sequences between serotype 6C and serotype 6D. On the other hand, serotypes 6A and 6D had the most highly similar wzy and wzx gene sequences. The wzy sequences of serotype 6C were nearly identical (99.6%) to those of serotype 6A clade II strains. In conclusion, we revealed the diversity of the genetic background and cps sequences in each pneumococcal serotype of serogroup 6. Pneumococcal serotype diversity might be attributable to complex serial mutation and recombination events. (C) 2014 Elsevier GmbH. All rights reserved.
机译：进行这项研究是为了更好地了解肺炎球菌血清型6A，68、6C和6D的遗传多样性和进化相关性。对1991年至2010年间从儿童收集的临床标本中的160个血清群6分离株进行了多基因座序列分型（MLST）。我们鉴定了38种序列类型（ST），包括5种克隆复合体和12个单例。尽管大多数ST局限于一种血清型，但某些ST却被两种血清型共享，而一个ST则被三种血清型共享。在eBURST分析中，许多血清型6A的ST与6C或6D血清型显示出遗传相关性。在我们临床样品的74个分离株和GenBank的36个分离株中分析了5种荚膜多糖（cps）基因-wchA，wciO，wciP，wzy和wzx。在分析5个cps基因的串联序列时，每种血清型都有几种谱型和进化枝。观察到血清型6A和6B之间以及血清型6C和6D之间小的遗传距离，而具有插入缺失序列的血清型6B形成了明显的进化枝。当比较血清型之间的各个cps基因时，血清型6C和血清型6D之间的wchA和wciO基因序列也有很高的相似性。另一方面，血清型6A和6D具有高度相似的wzy和wzx基因序列。血清型6C的wzy序列与血清型6A进化枝II菌株的wzy序列几乎相同（99.6％）。总之，我们揭示了血清群6的每种肺炎球菌血清型的遗传背景和cps序列的多样性。肺炎球菌的血清型多样性可能归因于复杂的系列突变和重组事件。 （C）2014 Elsevier GmbH。版权所有。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号