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首页> 外文期刊>International journal of molecular medicine >Interleukin-15 increases calcineurin expression in 3T3-L1 cells: possible involvement on in vivo adipocyte differentiation.
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Interleukin-15 increases calcineurin expression in 3T3-L1 cells: possible involvement on in vivo adipocyte differentiation.

机译：白介素-15增加3T3-L1细胞中钙调磷酸酶的表达：可能参与体内脂肪细胞分化。

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Different studies have revealed that the Ca2+-dependent serine/threonine phosphatase calcineurin is involved in the regulation of adipocyte differentiation. Calcineurin acts as a Ca2+-dependent molecular switch that negatively regulates the ability of 3T3-L1 cells to undergo adipocyte differentiation by preventing the expression of critical proadipogenic transcription factors. In this study we investigated the role of interleukin-15 (IL-15), a cytokine previously known to be involved in the control of fat accretion by adipose cells, in the differentiation of the 3T3-L1 preadipose cell line. We found that IL-15 is able to increase alpha-calcineurin mRNA content in white adipose tissue of rats chronically treated with the cytokine and also in the 3T3-L1 preadipose cell line. Moreover, IL-15 promoted a decrease in both leptin mRNA expression and lipid accumulation, as estimated by Red Oil O staining. Cotreatment with IL-15 and FK506 (a calcineurin inhibitor) resulted in no changes in lipid content compared with the non-treated group. These data suggest that IL-15 directly inhibits adipogenesis, possibly by upregulating alpha-calcineurin and preventing the induction of adipocyte differentiation.
机译：不同的研究表明，Ca2 +依赖性丝氨酸/苏氨酸磷酸酶钙调神经磷酸酶参与脂肪细胞分化的调控。钙调神经磷酸酶是一种依赖于Ca2 +的分子开关，可通过阻止关键的促脂肪形成转录因子的表达来负调控3T3-L1细胞经历脂肪细胞分化的能力。在这项研究中，我们研究了白细胞介素15（IL-15）（一种先前已知与脂肪细胞控制脂肪积聚有关的细胞因子）在3T3-L1脂肪细胞分化中的作用。我们发现，IL-15能够增加长期用细胞因子治疗的大鼠白色脂肪组织以及3T3-L1脂肪细胞系中α-钙调神经磷酸酶mRNA的含量。此外，IL-15促进了瘦素mRNA表达和脂质蓄积的减少，如Red Oil O染色所估计的。与未治疗组相比，与IL-15和FK506（钙调神经磷酸酶抑制剂）共同治疗不会导致脂质含量变化。这些数据表明，IL-15可能通过上调α-钙调神经磷酸酶和防止诱导脂肪细胞分化而直接抑制脂肪生成。

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