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首页> 外文期刊>Biochemical Pharmacology >Possible involvement of G(i3) protein in augmented contraction of bronchial smooth muscle from antigen-induced airway hyperresponsive rats.
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Possible involvement of G(i3) protein in augmented contraction of bronchial smooth muscle from antigen-induced airway hyperresponsive rats.

机译：G（i3）蛋白可能参与了抗原诱导的气道高反应性大鼠支气管平滑肌的收缩。

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摘要

To investigate a possible involvement of pertussis toxin (PTX)-sensitive heterotrimeric G proteins in the pathogenesis of airway hyperresponsiveness, the effect of PTX treatment on the augmented contractile response to acetylcholine (ACh) in bronchial smooth muscle of antigen-induced airway hyperresponsive rats was determined. In bronchial smooth muscle of airway hyperresponsive rats that were actively sensitized and repeatedly challenged with 2,4-dinitrophenylated Ascaris suum antigen, ACh-induced contractions were markedly augmented. The augmented contractile responses in the airway hyperresponsive group were significantly inhibited after treatment with PTX (1 microg/mL for 6 hr, 37 degrees ), whereas only a slight attenuation was observed in the normal control group. The level of G(alpha)i3 (measured by immunoblotting), but not other alpha-subunits of G(i/o) family proteins, in bronchial smooth muscle of the airway hyperresponsive rats was significantly increased as compared with that of control animals. It is concluded that PTX-sensitive muscarinic contractile responses of bronchial smooth muscle might be augmented upon antigen-induced airway hyperresponsiveness in rats, probably due to an up-regulation of G(alpha)i3 protein of bronchial smooth muscle.
机译：为了研究百日咳毒素（PTX）敏感异三聚体G蛋白可能与气道高反应性发病机制有关，PTX处理对抗原诱导的气道高反应性大鼠支气管平滑肌对乙酰胆碱（ACh）增强收缩反应的影响是决心。在气道高反应性大鼠的支气管平滑肌中，积极地对其进行敏化并反复受到2,4-二硝基苯基化的A虫抗原的攻击，ACh引起的收缩明显增强。气道高反应组的收缩反应增强在PTX治疗后（1 microg / mL，6小时，37度）被显着抑制，而在正常对照组中仅观察到轻微的衰减。与反应动物相比，气道高反应性大鼠支气管平滑肌中的Gα13（通过免疫印迹法测定）水平升高，但G（i / o）家族蛋白的其他α亚基水平未见明显升高。结论是，抗原诱导的大鼠气道高反应性可能会增强PTX敏感的支气管平滑肌毒蕈碱收缩反应，这可能是由于支气管平滑肌Gα13蛋白上调所致。

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