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首页> 外文期刊>International Journal of Zoology >Effect of Addition of Melatonin on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen
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Effect of Addition of Melatonin on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen

机译：褪黑激素的添加对密苏里子精液储液（5℃）的影响

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The present study was undertaken to assess the effect of melatonin (MT) on sperm motility, viability, total sperm abnormality, acrosomal and plasma membrane integrity, DNA abnormality, antioxidant profiles such as superoxide dismutase (SOD), catalase(CAT), glutathione (GSH) and total antioxidant capacity (TAC), enzymatic profiles such as aspartate amino transaminase (AST), alanine amino transaminase (ALT), and biochemical profiles such as malonaldehyde (MDA) production and cholesterol efflux. Totalnumbers of 30 ejaculates were collected twice a week from eight mithun bulls and semen was split into five equal aliquots, diluted with the TEYC extender. Group 1 has semen without additives (control) and group 2 to group 5 have semen that was diluted with 1 mM, 2 mM, 3 mM, and 4 mM of melatonin, respectively. These seminal parameters, antioxidant, enzymatic, and biochemical profiles were assessed at 5°C for 0, 6, 12, 24, and 30 h of incubation. Inclusion of melatonin into diluent resulted in significant (P < 0.05) decrease in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities at different hours of storage periods as compared with control group. Additionally, melatonin at 3 mM has significant improvement in quality of mithun semen than melatonin at 1 mM, 2 mM or 4 mM stored in in vitro for up to 30 h. It was concluded that the possible protective effects of melatonin on sperm parameters are it prevents MDA production and preserve the antioxidants and intracellular enzymes during preservation.
机译：本研究旨在评估褪黑激素（MT）对精子活力，生存力，总精子异常，顶体和质膜完整性，DNA异常，抗氧化剂谱如超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽（ GSH）和总抗氧化剂能力（TAC），酶学特征（例如天冬氨酸氨基转氨酶（AST），丙氨酸氨基转氨酶（ALT））和生化特征（例如丙二醛（MDA）产生和胆固醇外排）。每周两次从八只头公牛中收集总数为30的精液，将精液分成五等份，用TEYC增量剂稀释。第1组的精液中没有添加添加剂（对照），第2组至第5组的精液中分别用1 mM，2 mM，3 mM和4 mM的褪黑素稀释。在0、6、12、24和30 h的孵育温度下，于5°C评估这些重要参数，抗氧化剂，酶和生化特性。与对照组相比，在稀释剂中加入褪黑激素可导致在不同的贮藏时间，精子死亡，精子异常和顶体异常的百分比显着降低（P <0.05）。另外，3 mM的褪黑素比体外保存长达30 h的1 mM，2 mM或4 mM的褪黑素在丝裂精液质量上有显着改善。结论是褪黑激素对精子参数的可能的保护作用是它可以防止MDA的产生并在保存过程中保存抗氧化剂和细胞内酶。

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