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首页> 外文期刊>Iranian journal of public health. >Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens
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Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens

机译：快速，直接检测脑脊髓液标本中脑膜炎奈瑟氏菌的特异性PCR分析

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摘要

Background: Neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. The aim of this study was to evaluate a PCR-based assay for specific and rapid detection of N. meninigitidis in CSF specimens.Methods: Since April 2002 to July 2006, 130 CSF specimens were collected from patients suspected of having bacterial meningitis. Bacterial isolation and identification was carried out according to the standard bacteriological methods. The PCR was used to amplify a l01bp fragment of capsular transport gene A (ctrA) of N. meningitidis.Results: PCR yielded an amplified product with the expected size of 101 base pair fragment. Sensitivity test proved 500 ng of N. meningitidis DNA as the final detection limit and specificity test revealed no cross-reaction for a wide range of respiratory pathogenic organisms.Conclusion: The PCR assay was more sensitive than the bacterial culturing. It might be possible to apply this procedure for rapid diagnosis of meningococci in clinical samples.
机译：背景：脑膜炎奈瑟菌是与中枢神经系统感染相关的最常见微生物之一。这项研究的目的是评估一种基于PCR的检测方法，用于特异性和快速地检测脑脊液标本中的脑膜炎奈瑟菌。方法：自2002年4月至2006年7月，从怀疑患有细菌性脑膜炎的患者中收集了130份脑脊液标本。细菌的分离和鉴定是根据标准的细菌学方法进行的。 PCR扩增脑膜炎奈瑟氏球菌荚膜转运基因A（ctrA）的10bp片段。结果：PCR扩增出预期大小为101个碱基对的片段。敏感性测试证明500 ng脑膜炎奈瑟氏菌DNA为最终检测极限，特异性测试显示与多种呼吸道致病菌无交叉反应。结论：PCR检测比细菌培养更敏感。可以将此方法用于临床样品中脑膜炎球菌的快速诊断。

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