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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Recent advances in CE-mediated microanalysis for enzyme study
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Recent advances in CE-mediated microanalysis for enzyme study

机译:CE介导的酶分析技术的最新进展

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摘要

This review gives an overview of the recent developments and applications in the use of CE-mediated microanalysis for enzyme studies. The period covers mid-2011 until mid-2013. Both off-line and in-line enzyme assays with their applications using CE are described in this article. For the in-capillary enzyme reaction, the techniques using electrophoretically mediated microanalysis (EMMA) as well as immobilized enzyme reactor (IMER) are discussed. The applications include the evaluation of enzyme activity, enzyme kinetics, enzyme inhibition, screening of enzyme inhibitors, and the study of enzyme-mediated drug metabolism.
机译:这篇综述概述了使用CE介导的微量分析进行酶研究的最新进展和应用。该期间为2011年中至2013年中。本文介绍了使用CE的离线和在线酶分析方法及其应用。对于毛细管内酶反应,讨论了使用电泳介导的微量分析(EMMA)和固定化酶反应器(IMER)的技术。应用包括评估酶活性,酶动力学,酶抑制,酶抑制剂的筛选以及酶介导的药物代谢研究。

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