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首页> 外文期刊>Endocrinology >Evolutionary history and functional characterization of androgen receptor genes in jawed vertebrates.
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Evolutionary history and functional characterization of androgen receptor genes in jawed vertebrates.

机译：颌骨脊椎动物中雄激素受体基因的进化历史和功能表征。

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摘要

Vertebrates show diverse sexual characters in sexually attractive and reproductive organs, which are regulated by steroid hormones, particularly androgens. However, the evolutionary history of androgen receptor (AR) gene remains largely unknown on the basis of phylogenic and functional analyses. To elucidate the evolutionary history and functional diversification of AR genes in vertebrates, we cloned the AR cDNAs from a shark, basal ray-finned fishes (Actinopterygii), namely bichir and sturgeon (Acipenseriformes), and teleosts including a basal teleost, arowana (Osteoglossiformes). Molecular phylogenetic analysis revealed that the gene duplication event that gave rise to two different teleost ARs (alpha and beta) likely occurred in the actinopterygian lineage leading to teleosts after the divergence of Acipenseriformes but before the split of Osteoglossiformes, which is compatible with the phylogenetic timing of teleost-specific genome duplication. Searching for AR genes in the medaka genome indicated that the teleost AR gene duplication has been associated with the duplication between chromosomes 10 and 14. Our functional analysis revealed that the shark AR activates the target gene via androgen response element by classical androgens. The teleost ARalpha showed the unique intracellular localization with a significantly higher transactivating capacity than that by teleost ARbeta. These findings indicate that the most ancient type of AR, as activated by the classical androgens as ligands, emerged before the Chondrichthyes-Osteichthyes split, and the AR gene was duplicated during the teleost-specific genome duplication event. We report here for the first time the accurate evolutionary history of AR gene and functional characterization of AR duplicates in teleost lineage.
机译：脊椎动物在性吸引和生殖器官中显示出多种性征，这些性征受类固醇激素，尤其是雄激素的调节。但是，基于系统发育和功能分析，雄激素受体（AR）基因的进化史仍然未知。为了阐明脊椎动物中AR基因的进化历史和功能多样化，我们从鲨鱼，基鳍鳍鱼（Actinopterygii）（即碧hir鱼和st鱼）和硬骨鱼（包括基底硬骨鱼，龙鱼（Osteoglossiformes））中克隆了AR cDNA。 ）。分子系统发育分析表明，引起两个不同的硬骨鱼类AR（α和β）的基因复制事件可能发生在放线翅目谱系中，从而导致硬骨鱼类在Acipenseriformes发散之后但在骨骨类动物分裂之前发生，这与系统发生的时间相吻合。硬骨鱼类特异的基因组复制。在medaka基因组中搜索AR基因表明，硬骨鱼AR基因重复与10号染色体和14号染色体之间的重复有关。我们的功能分析表明，鲨鱼AR通过经典的雄激素通过雄激素反应元件激活靶基因。硬骨鱼ARalpha显示出独特的细胞内定位，其转激活能力明显高于硬骨鱼ARbeta。这些发现表明，由经典雄激素作为配体激活的最古老的AR类型出现在软骨鱼类-硬骨鱼类分裂之前，AR基因在硬骨鱼特异性基因组复制过程中被复制。我们在这里第一次报告硬骨质谱系中AR基因的准确进化史和AR复制品的功能表征。

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