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首页> 外文期刊>European Journal of Histochemistry >Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation
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Delayed maturation and altered proliferation within the rat rostral migratory stream following maternal deprivation

机译：母体剥夺后大鼠延髓mi游流的延迟成熟和增殖改变

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摘要

The objective of this study was to investigate whether stressful experience during early postnatal period may influence morphological characteristics of the rat neurogenic pathway - the rostral migratory stream (RMS) and proliferation of neuronal precursors in three successive areas of the RMS: in the vertical arm, the elbow and the horizontal arm. To induce stress, the pups were subjected to repeated maternal deprivation during the first postnatal week after birth. Brains were analyzed at the seventh postnatal day. The controls matched the age of maternally deprived animals. Observation of hematoxylin-eosin stained sections showed that maternal deprivation did not affect the general morphological appearance of the RMS. The shape of the RMS of maternally deprived rats resembles the RMS of control animals. Maternal deprivation caused slight, not significant increase in the RMS thickness in comparison with control rats. Significant difference between the control and maternally deprived rats concerns the olfactory ventricle. While in seven days old control rats the olfactory ventricle is completely closed, in maternally deprived rats of the same age the olfactory ventricle was regularly visible as a narrow lumen at the axis of the RMS horizontal arm. This finding indicates delayed maturation of the migratory pathway as a consequence of stress. Proliferation activity has been assessed by immunoreactivity of the endogenous cell cycle protein Ki-67. The results of Ki-67 immunohistochemistry showed that seven days' maternal separation for 3 h daily induces significant quantitative changes in the number of proliferating cells within the RMS. The response of Ki- 67-positive cells to stress differed in individual part of the RMS, with a marked decrease in the vertical arm and a significant increase in the elbow, suggesting heterogeneity of neural stem cells along the RMS; while in the RMS vertical arm the number of dividing cells significantly decreased, there was a marked increase of Ki-67-positive cells in the RMS elbow. This suggests heterogeneity of neural stem cells along the RMS.
机译：这项研究的目的是调查产后早期的压力经历是否会影响大鼠神经源性通路的形态特征-RMS的三个连续区域的鼻侧迁徙流（RMS）和神经元前体的增殖：在垂直臂上，肘部和横臂。为了诱发压力，幼犬在出生后的第一个产后一周内反复遭受母体剥夺。在出生后第七天对大脑进行分析。对照与母体剥夺动物的年龄相匹配。苏木精-伊红染色切片的观察表明，母体剥夺并不影响RMS的一般形态。母体剥夺大鼠的RMS形状类似于对照动物的RMS。与对照组相比，母体剥夺导致RMS厚度略有增加，但没有明显增加。对照组和母体剥夺的大鼠之间的显着差异涉及嗅觉心室。在7天大的对照组大鼠中，嗅觉室完全关闭，而在同龄的母体剥夺的大鼠中，嗅觉室通常在RMS水平臂的轴上可见为狭窄的管腔。该发现表明，由于压力，迁移途径的延迟成熟。已经通过内源性细胞周期蛋白Ki-67的免疫反应性评估了增殖活性。 Ki-67免疫组织化学的结果表明，母体分离每天7天，每天3小时，导致RMS内增殖细胞数量发生明显的定量变化。 Ki-67阳性细胞对应力的反应在RMS的各个部分中有所不同，垂直臂的显着减少和肘部的显着增加，表明神经干细胞沿RMS的异质性。而在RMS垂直臂中，分裂细胞的数量显着减少，RMS肘部的Ki-67阳性细胞显着增加。这表明沿RMS的神经干细胞的异质性。

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