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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >Absence of coupling between release and biosynthesis of peptide hormonesin insect neuroendocrine cells
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Absence of coupling between release and biosynthesis of peptide hormonesin insect neuroendocrine cells

机译：昆虫神经内分泌细胞中肽激素的释放与生物合成之间缺乏耦合

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摘要

Adipokinetic hormone (AKH)-producing cells in the corpus cardiacum of the insect Locusta migratoria represent a neuroendocrine system containing large quantities of stored secretory peptides. In the present study we address the question whether the release of AKHs from these cells induces a concomitant enhancement of their biosynthesis. The effects of hormone release in vivo (by flight activity) and in vitro (using crustacean cardioactive peptide, locustamyoinhibiting peptide, and activation of protein kinase A and C) on the biosynthetic activity for AKHs were measured. The intracellular levels of prepro-AKH mRNAs, the intracellular levels of pro-AKHs, and the rate of synthesis of (pro-)AKHs were used as parameters for biosynthetic activity. The effectiveness of in vitro treatment was assessed from the amounts of AKHs released. Neither flight activity as the natural stimulus for AKH release, nor in vitro treatment with the regulatory peptides or signal transduction activators appeared to affect the biosynthetic activity for AKHs. This points to an absence of coupling between release and biosynthesis of AKHs. The strategy of the AKH-producing cells to cope with variations in secretory stimulation seems to rely on a pool of secretory material that is readily releasable and continuously replenished by a process of steady biosynthesis.
机译：昆虫蝗的心体中产生脂肪动力学激素（AKH）的细胞代表含有大量储存的分泌肽的神经内分泌系统。在本研究中，我们解决了以下问题：AKHs从这些细胞的释放是否会诱导其生物合成的同时增强。测量了体内（通过飞行活性）和体外（使用甲壳类心脏活性肽，基因定位抑制性肽以及蛋白激酶A和C的激活）激素释放对AKHs生物合成活性的影响。前pro-AKH mRNA的细胞内水平，pro-AKHs的细胞内水平和（pro-）AKHs的合成速率被用作生物合成活性的参数。从释放的AKHs的量评估了体外治疗的有效性。飞行活动不是AKH释放的自然刺激，也不是用调节肽或信号转导激活剂进行的体外治疗都不会影响AKH的生物合成活性。这表明AKH的释放和生物合成之间不存在偶联。产生AKH的细胞应对分泌刺激变化的策略似乎依赖于分泌物质的集合，该集合易于通过稳定的生物合成过程释放并不断补充。

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