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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Effects of complement regulators bound to Escherichia coli K1 and Group B Streptococcus on the interaction with host cells.
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Effects of complement regulators bound to Escherichia coli K1 and Group B Streptococcus on the interaction with host cells.

机译：与大肠杆菌K1和B组链球菌结合的补体调节剂对与宿主细胞相互作用的影响。

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Escherichia coli K1 and Group B Streptococcus (GBS) are the most common bacteria that cause meningitis during the neonatal period. Complement, the first line of defence in the host, acts on these bacteria to opsonize with various components of complement for subsequent presentation to phagocytes. To counteract these opsonization effects, E. coli and GBS bind to the complement regulators C4 binding protein and Factor H, respectively. Nonetheless, the deposition of complement components on these two bacteria from neonatal serum and their effect on the host cell interaction is unclear. Here we demonstrated that the deposition of complement proteins from adult serum prevented the invasion of E. coli into human brain microvascular endothelial cells, whereas the invasion of GBS was enhanced. In contrast, treatment with cord serum had no effect on the invasion of both these bacteria. We also examined the effect of the deposited complement proteins on phagocytosis using THP-1 cells and THP-1 cells differentiated into macrophages. Escherichia coli treated with adult serum neither attached nor entered these cells, whereas GBS was phagocytosed and survived efficiently. We further demonstrate that the inhibitory effect of complement proteins is the result of the bound complement inhibitors C4b-binding protein, in the case of E. coli, and Factor H, in the case of GBS. Taken together, these results suggest that E. coli and GBS utilize contrasting mechanisms of complement-mediated interactions with their target cells for successful establishment of disease.
机译：大肠杆菌K1和B组链球菌（GBS）是在新生儿期引起脑膜炎的最常见细菌。补体是宿主的第一道防线，作用于这些细菌，与补体的各种成分发生调理作用，随后呈现给吞噬细胞。为了抵消这些调理作用，大肠杆菌和GBS分别与补体调节剂C4结合蛋白和因子H结合。然而，尚不清楚新生儿血清中这两种细菌中补体成分的沉积及其对宿主细胞相互作用的影响。在这里，我们证明了成人血清中补体蛋白的沉积阻止了大肠杆菌向人脑微血管内皮细胞的侵袭，而GBS的侵袭得到了增强。相反，用脐带血清处理对这两种细菌的​​入侵均没有影响。我们还检查了使用THP-1细胞和分化为巨噬细胞的THP-1细胞对补体蛋白的吞噬作用。用成人血清处理的大肠杆菌既不附着也不进入这些细胞，而GBS被吞噬并有效存活。我们进一步证明，补体蛋白的抑制作用是结合的补体抑制剂C4b结合蛋白（对于大肠杆菌）和因子H（对于GBS）的结果。综上所述，这些结果表明，大肠杆菌和GBS利用补体介导的与其靶细胞相互作用的对比机制来成功建立疾病。

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