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Genome-wide identification of lineage-specific genes in Arabidopsis, Oryza and Populus.

机译:全基因组鉴定的拟南芥,稻和胡杨的谱系特异性基因。

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摘要

Protein sequences were compared among Arabidopsis, Oryza and Populus to identify differential gene (DG) sets that are in one but not the other two genomes. The DG sets were screened against a plant transcript database, the NR protein database and six newly-sequenced genomes (Carica, Glycine, Medicago, Sorghum, Vitis and Zea) to identify a set of species-specific genes (SS). Gene expression, protein motif and intron number were examined. 165, 638 and 109 SS genes were identified in Arabidopsis, Oryza and Populus, respectively. Some SS genes were preferentially expressed in flowers, roots, xylem and cambium or up-regulated by stress. Six conserved motifs in Arabidopsis and OryzaSS proteins were found in other distant lineages. The SS gene sets were enriched with intronless genes. The results reflect functional and/or anatomical differences between monocots and eudicots or between herbaceous and woody plants. The Populus-specific genes are candidates for carbon sequestration and biofuel research.
机译:比较了拟南芥,稻和胡杨中的蛋白质序列,以鉴定在一个基因组中而不在其他两个基因组中的差异基因(DG)集。根据植物转录本数据库,NR蛋白数据库和六个新测序的基因组(Carica,Glycine,Medicago,高粱,Vitis和Zea)筛选DG集,以鉴定一组物种特异性基因(SS)。检查基因表达,蛋白质基序和内含子数。在拟南芥,稻和胡杨中分别鉴定出165、638和109个SS基因。一些SS基因优先在花,根,木质部和形成层中表达或通过胁迫上调。在其他遥远的世系中发现了拟南芥和OryzaSS蛋白中的六个保守基序。 SS基因集富含无内含子基因。结果反映了单子叶植物和双子叶植物之间或草本植物和木本植物之间的功能和/或解剖学差异。胡杨特有的基因是碳固存和生物燃料研究的候选者。

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