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Expression profile of FGF receptors in preimplantation ovine embryos and the effect of FGF2 and PD173074

机译：FGF受体在植入前绵羊胚胎中的表达谱及FGF2和PD173074的作用

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Fibroblast growth factors (FGFs) and their receptors (FGFRs) are increasingly recognized as important regulators of embryo development in mammals. This study investigated the importance of FGF signaling during in vitro development of ovine embryo. The mRNAs of four FGFR subtypes were detected throughout preimplantation development of in vitro fertilized (IVF) embryos, peaked in abundance at the morula stage, and decreased significantly at the blastocyst stage. To gain insight into the role of these mRNAs in embryo development, IVF embryos were cultured in the presence of FGF2 (100 or 500ng/ml: beginning from days 1 or 4 to 7) or PD173074 (1 mu M: beginning from days 1 to 7) as usual treatments for activation or inhibition of FGFRs, respectively. FGF2-supplementation did not affect the percentage of embryos that developed to the blastocyst, blastocyst cell count and the proportion of cells allocated in inner cell mass (ICM) and trophectoderm (TE) compared to control (p>0.05). Also, increasing the dosage or duration of FGF2 treatment did not significantly alter blastocyst yield or differential cell count (p>0.05). PD173074-mediated inhibition of FGFRs did not significantly affect blastocyst yield (p>0.05). Assessment of expression profiles of lineage-associated markers revealed that FGF2 (500ng/ml) supplementation: (i) significantly increased expression of putative hypoblast marker (GATA4), (ii) significantly decreased expression of putative epiblast (EPI) marker (NANOG) and (iii) did not change TE markers (CDX2 and IFNT) and pluripotency makers (OCT4, SOX2 and REX1). In summary, FGF2-mediated activation of FGFRs may promote a switch in transcriptional profile of ovine ICM from EPI- to hypoblast-associated gene expression.
机译：成纤维细胞生长因子（FGFs）及其受体（FGFRs）越来越被认为是哺乳动物胚胎发育的重要调节剂。这项研究调查了在绵羊胚胎体外发育过程中FGF信号传导的重要性。在体外受精（IVF）胚胎植入前的整个发育过程中检测到四种FGFR亚型的mRNA，在桑ula期达到最高峰，在胚泡期则显着下降。为了深入了解这些mRNA在胚胎发育中的作用，在FGF2（100或500ng / ml：从第1或4至7天开始）或PD173074（1μM：从第1至4天开始）的条件下培养IVF胚胎。 7）分别为激活或抑制FGFR的常规治疗。与对照组相比，补充FGF2不会影响发育为胚泡的胚百分比，胚泡细胞数以及分配在内部细胞团（ICM）和滋养外胚层（TE）中的细胞比例（p> 0.05）。同样，增加FGF2治疗的剂量或持续时间也不会显着改变胚泡产量或差异细胞计数（p> 0.05）。 PD173074介导的FGFR抑制不显着影响胚泡产量（p> 0.05）。评估谱系相关标记的表达谱表明，FGF2（500ng / ml）的补充：（i）显着增加了假定的次胚标记（GATA4）的表达，（ii）显着减少了上皮细胞（EPI）的表达（NANOG）和（iii）不会改变TE标记（CDX2和IFNT）和多能性产生者（OCT4，SOX2和REX1）。总而言之，FGF2介导的FGFR激活可能促进绵羊ICM转录模式从EPI-转变为与次胚相关的基因表达。

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